Human Neurokinin B ELISA Kit (Colorimetric)

ELISA: Human Neurokinin B ELISA Kit (Colorimetric) [NBP2-76743] - Samples were spiked with high concentrations of Human Neurokinin B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Neurokinin B ELISA Kit (Colorimetric) [NBP2-76743] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.625 - 1000pg/mL
• Sensitivity: 9.375pg/mL

Human Neurokinin B ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 15.625 - 1000pg/mL
• Sensitivity: 9.375pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 5.38
• Intra-Assay: CV% < 4.66
• Spike Recovery: 90-111%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TAC3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Neurokinin B ELISA Kit (Colorimetric)

• gamma tachykinin 3
• neurokinin beta
• neurokinin B-like
• neuromedin K
• preprotachykinin B
• PRO1155
• tachykinin 3
• tachykinin-3
• ZNEUROK1NKNBNKB

Background

The Neurokinins, also known as Tachykinins, belong to an evolutionary conserved family of peptide neurotransmitters that share a c-terminal sequence and have an established role in neurotransmission. The mammalian tachykinins include substance P (NK1), neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma.

Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Neurokinin B ELISA Kit (NBP2-76743)(1)

Publication using NBP2-76743

• Zhang L, Fernando T, Liu Y et al. Neurokinin 3 receptor antagonist-induced adipocyte activation improves obesity and metabolism in PCOS-like mice Life sciences 2022-10-14 [PMID: 36252700] (ELISA)

FAQs for Neurokinin B ELISA Kit (NBP2-76743). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TAC3