Neurofibromin 1 Antibody (McNFn27a)

Images

 
Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.
Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, GPSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clone
McNFn27a
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Neurofibromin 1 Antibody (McNFn27a) Summary

Immunogen
A highly conserved N-terminal peptide, corresponding to amino acids 27-41 of human Neurofibromin 1. [UniProt# P21359]
Localization
Cytoplasmic
Specificity
This is specific for full-length or truncated forms of neurofibromin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Immunoprecipitation 1:10-1:500
Application Notes
This Neurofibromin 1 (McNFn27a) antibody is useful for ELISA, Western blot and Immunoprecipitation. Immunohistochemistry-Paraffin was reported in scientific literature.
Publications
Read Publication using
NB300-153 in the following applications:

Reactivity Notes

Human and rodent.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Neurofibromin 1 Antibody (McNFn27a)

  • DKFZp686J1293
  • FLJ21220
  • Neurofibromatosis-related protein NF-1
  • neurofibromin 1
  • neurofibromin
  • NFNS
  • VRNF
  • WSS

Background

Neurofibromin 1 (NF1), the Nf1 gene product, is a large protein which contains a central Ras-GTPase-activating (RasGAP) domain and is suggested to function as a negative regulator of proto-oncogene RAS and downstream effectors. It stimulates the GTPase activity of RAS and acts as regulator of RAS signaling. Consistent with its biochemical activity, NF1 functions as a tumor-suppressor gene in juvenile myelomonocytic leukemia (JMML), and diseased BM cells exhibit aberrant Raf/MEK/ERK signaling. Moreover, NF1 shows greater affinity for Ras GAP, but lower specific activity. Besides JMML, defects in NF1 have also been linked to neurofibromatosis type 1 (NF1), Watson syndrome (WS), familial spinal neurofibromatosis (FSNF), neurofibromatosis-Noonan syndrome (NFNS), colorectal cancer (CRC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurofibromin 1 Antibody (NB300-153)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Neurofibromin 1 Antibody (NB300-153) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-153AF405
Alexa Fluor 488 Labeled NB300-153AF488
Alexa Fluor 647 Labeled NB300-153AF647
Alexa Fluor 700 Labeled NB300-153AF700
Biotin Labeled NB300-153B
DyLight 405 Labeled NB300-153V
DyLight 488 Labeled NB300-153G
DyLight 550 Labeled NB300-153R
DyLight 650 Labeled NB300-153C
FITC Labeled NB300-153F
HRP Labeled NB300-153H

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Bioinformatics Tool for Neurofibromin 1 Antibody (NB300-153)

Discover related pathways, diseases and genes to Neurofibromin 1 Antibody (NB300-153). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Neurofibromin 1.

Neurofibromatosis Infographic
Neurofibromatosis (NF) is a genetic disorder caused by mutations in the NF1, NF2 or SMARCB1 genes which lead to tumor growth on nerves throughout the body. Although the tumors are usually benign, they still require chemotherapy to shrink and may becom...  Read full blog post.

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Bioinformatics

Gene Symbol NF1