NeuroD2 Antibody (PCRP-NEUROD2-1G1) - Azide and BSA Free

Protein Array: NeuroD2 Antibody (PCRP-NEUROD2-1G1) - Azide and BSA Free [NBP3-14015] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NeuroD2 Antibody (PCRP-NEUROD2-1G1).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NeuroD2 Antibody (PCRP-NEUROD2-1G1) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow cytometric analysis of PFA-fixed HeLa cells. NeuroD2 Antibody (PCRP-NEUROD2-1G1) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NeuroD2 Antibody (PCRP-NEUROD2-1G1) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-NEUROD2-1G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

NeuroD2 Antibody (PCRP-NEUROD2-1G1) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13981).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human NeuroD2 protein (Uniprot: Q15784)
• Localization: Nucleus
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEUROD2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Protein Array
• Application Notes: ELISA: For coating, order this antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for NeuroD2 Antibody (PCRP-NEUROD2-1G1) - Azide and BSA Free

• BHLHA1
• bHLHa1neurogenic differentiation factor 2
• Class A basic helix-loop-helix protein 1
• MGC26304
• NDRF
• NDRFneurogenic basic-helix-loop-helix protein
• NeuroD2
• NeuroD-related factor
• neurogenic differentiation 2

Background

The NeuroD2 gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEUROD2