Neurexin 3/NRXN3 Antibody

Product Discontinued

Neurexin 3/NRXN3 Antibody Summary

• Immunogen: Peptide with sequence C-PQERKDARSQKNT corresponding to internal region according to NP_004787.2.
• Epitope: C terminus
• Specificity: This antibody is expected to recognize the reported isoform 1 (NP_004787.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRXN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments gave an approx. 24 kDa band in human brain (cerebellum) lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 117 kDa band according to NP_004787.2. The 24 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Neurexin 3/NRXN3 Antibody

• C14orf60
• chromosome 14 open reading frame 60
• KIAA0743MGC176711
• Neurexin 3
• neurexin III
• Neurexin III-alpha
• Neurexin III-beta
• neurexin-3-beta
• NRXN3

Background

Neurexins are a family of proteins that function in the vertebrate nervous system as cell adhesion molecules and receptors. They are encoded by several unlinked genes of which two, NRXN1 and NRXN3, are among the largest known human genes. Three of the genes (NRXN1-3) utilize two alternate promoters and include numerous alternatively spliced exons to generate thousands of distinct mRNA transcripts and protein isoforms. The majority of transcripts are produced from the upstream promoter and encode alpha-neurexin isoforms; a much smaller number of transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. The alpha-neurexins contain epidermal growth factor-like (EGF-like) sequences and laminin G domains, and have been shown to interact with neurexophilins. The beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurexin 3/NRXN3 Antibody (NBP1-28485)(1)

Publication using NBP1-28485

• Vieira AR, McHenry TG, Daack-Hirsch S et al. Candidate gene/loci studies in cleft lip/palate and dental anomalies finds novel susceptibility genes for clefts.susceptibility genes for clefts. Genet Med 2008-09-01 [PMID: 18978678]

Bioinformatics

• Gene Symbol: NRXN3
• Entrez:
  • 9369(Human)
  • 18191(Mouse)
• Uniprot:
  • NP_004787.2(Human)