Neurexin 3/NRXN3 Antibody

Images

 
Western blot shows lysates of mouse brain tissue and SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Neurexin 3/NRXN3 Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Neurexin 3/NRXN3 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neurexin 3/NRXN3
Ser35-Thr357
Accession # NP_620426
Specificity
Detects human and mouse Neurexin 3/NRXN3 in Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Neurexin 1 beta is observed and less than 5% cross-reactivity recombinant rat (rr) Neurexin 1 alpha and rrNeurexin 2 beta is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NRXN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurexin 3/NRXN3 Antibody

  • C14orf60
  • chromosome 14 open reading frame 60
  • KIAA0743MGC176711
  • Neurexin 3
  • neurexin III
  • Neurexin III-alpha
  • Neurexin III-beta
  • neurexin-3-beta
  • NRXN3

Background

The alpha and beta forms of Neurexins 1‑3 are transmembrane neuronal glycoproteins which are transcribed from each of three NRXN genes that utilize alternate promoters. Like other Neurexins, the extracellular domain (ECD) of Neurexin 3 alpha contains six LNS domains interspersed with three EGF-like domains, while that of Neurexin 3 beta contains only the sixth LNS domain and no EGF-like domains (1‑3). Mature human Neurexin 3 beta is a 70 kDa glycosylated protein with a 528 amino acid (aa) ECD and a 56 aa cytoplasmic domain that contains a motif for binding PDZ scaffolding proteins (3‑5). Within comparable regions of the ECD, human Neurexin 3 beta shares 99% aa sequence identity with mouse and rat Neurexin 3 beta. It shares 65% aa sequence identity with comparable regions of the ECD of human Neurexin 1 beta and 2 beta. Alternative splicing of human Neurexin 3 beta generates multiple isoforms. There are potentially soluble and secreted variants and some which contain a fibronectin type III-like domain (4, 6). Neurexin 3 beta is widely expressed in the brain where it binds the postsynaptic Neuroligins 1, 2, and 3 (6‑9). Neurexin 3 beta may also be expressed in non-nervous tissues with a potentially cardiac-specific isoform (10). Human Neurexin 3 beta polymorphisms which affect the splicing pattern are associated with susceptibility to alcohol dependence (6). The Neurexin 3 beta genetic locus has been linked to opioid and nicotine addiction, and Neurexin 3 beta gene expression is up‑regulated after short term exposure of mice to cocaine (11‑13).

  1. Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.
  2. Dean, C. and T. Dresbach (2006) Trends Neurosci. 29:21.
  3. Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833.
  4. Rowen, L. et al. (2002) Genomics 79:587.
  5. Ushkaryov, Y. A. et al. (1994) J. Biol. Chem. 269:11987.
  6. Hishimoto, A. et al. (2007) Hum. Mol. Genet. 16:2880.
  7. Ullrich, B. et al. (1995) Neuron 14:497.
  8. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  9. Ichtchenko, K. et al. (1995) Cell 81:435.
  10. Occhi, G. et al. (2002) Biochem. Biophys. Res. Commun. 298:151.
  11. Lachman, H.M. et al. (2007) Hum. Mol. Genet. 16:1327.
  12. Bierut, L.J. et al. (2007) Hum. Mol. Genet. 16:24.
  13. Kelai, S. et al. (2008) Neuroreport 19:751.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF4524
Species: Rt
Applications: WB
NB110-60935
Species: Hu, Mu, Pm
Applications: WB
NBP2-41103
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-90080
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-87456
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF5634
Species: Hu
Applications: WB
NBP1-92090
Species: Hu
Applications: Simple Western, ICC/IF, IHC, IHC-P
NBP1-90558
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF5645
Species: Hu, Rt
Applications: WB
NBP1-77122
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
248-BD
Species: Hu, Mu, Rt, Ca, Eq
AF4340
Species: Hu, Rt
Applications: WB, IHC
NBP1-76997
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
NBP1-83116
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-76998
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-24713
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, IHC-P
NBP2-14148
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF4076
Species: Mu, Rt
Applications: WB, Simple Western, Flow, CyTOF-ready, ICC
NBP2-66296
Species: Hu

Publications for Neurexin 3/NRXN3 Antibody (AF5269) (0)

There are no publications for Neurexin 3/NRXN3 Antibody (AF5269).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Neurexin 3/NRXN3 Antibody (AF5269) (0)

There are no reviews for Neurexin 3/NRXN3 Antibody (AF5269). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Neurexin 3/NRXN3 Antibody (AF5269) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Neurexin 3/NRXN3 Antibody (AF5269)

Discover related pathways, diseases and genes to Neurexin 3/NRXN3 Antibody (AF5269). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Neurexin 3/NRXN3 Antibody (AF5269)

Discover more about diseases related to Neurexin 3/NRXN3 Antibody (AF5269).
 

Pathways for Neurexin 3/NRXN3 Antibody (AF5269)

View related products by pathway.

Blogs on Neurexin 3/NRXN3

There are no specific blogs for Neurexin 3/NRXN3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Neurexin 3/NRXN3 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NRXN3
Entrez
Uniprot