Neurexin 3/NRXN3 Antibody [Unconjugated]

Neurexin 3/NRXN3 was detected in immersion fixed paraffin-embedded sections of human brain cerebellum using Sheep Anti-Human/Mouse Neurexin 3/NRXN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5269) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje Neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western blot shows lysates of Mouse Brain. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse Neurexin 3/NRXN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurexin 3/NRXN3 at approximately ~150 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Neurexin 3/NRXN3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Neurexin 3/NRXN3
  Ser35-Thr357
  Accession # NP_620426
• Specificity: Detects human and mouse Neurexin 3/NRXN3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NRXN3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurexin 3/NRXN3 Antibody [Unconjugated]

• C14orf60
• chromosome 14 open reading frame 60
• KIAA0743MGC176711
• Neurexin 3
• neurexin III
• Neurexin III-alpha
• Neurexin III-beta
• neurexin-3-beta
• NRXN3

Background

The alpha and beta forms of Neurexins 1‑3 are transmembrane neuronal glycoproteins which are transcribed from each of three NRXN genes that utilize alternate promoters. Like other Neurexins, the extracellular domain (ECD) of Neurexin 3 alpha contains six LNS domains interspersed with three EGF-like domains, while that of Neurexin 3 beta contains only the sixth LNS domain and no EGF-like domains (1‑3). Mature human Neurexin 3 beta is a 70 kDa glycosylated protein with a 528 amino acid (aa) ECD and a 56 aa cytoplasmic domain that contains a motif for binding PDZ scaffolding proteins (3‑5). Within comparable regions of the ECD, human Neurexin 3 beta shares 99% aa sequence identity with mouse and rat Neurexin 3 beta . It shares 65% aa sequence identity with comparable regions of the ECD of human Neurexin 1 beta and 2 beta . Alternative splicing of human Neurexin 3 beta generates multiple isoforms. There are potentially soluble and secreted variants and some which contain a fibronectin type III-like domain (4, 6). Neurexin 3 beta is widely expressed in the brain where it binds the postsynaptic Neuroligins 1, 2, and 3 (6‑9). Neurexin 3 beta may also be expressed in non-nervous tissues with a potentially cardiac-specific isoform (10). Human Neurexin 3 beta polymorphisms which affect the splicing pattern are associated with susceptibility to alcohol dependence (6). The Neurexin 3 beta genetic locus has been linked to opioid and nicotine addiction, and Neurexin 3 beta gene expression is up‑regulated after short term exposure of mice to cocaine (11‑13).

1. Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.
2. Dean, C. and T. Dresbach (2006) Trends Neurosci. 29:21.
3. Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833.
4. Rowen, L. et al. (2002) Genomics 79:587.
5. Ushkaryov, Y. A. et al. (1994) J. Biol. Chem. 269:11987.
6. Hishimoto, A. et al. (2007) Hum. Mol. Genet. 16:2880.
7. Ullrich, B. et al. (1995) Neuron 14:497.
8. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
9. Ichtchenko, K. et al. (1995) Cell 81:435.
10. Occhi, G. et al. (2002) Biochem. Biophys. Res. Commun. 298:151.
11. Lachman, H.M. et al. (2007) Hum. Mol. Genet. 16:1327.
12. Bierut, L.J. et al. (2007) Hum. Mol. Genet. 16:24.
13. Kelai, S. et al. (2008) Neuroreport 19:751.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurexin 3/NRXN3 Antibody (AF5269)(2)

Publications using AF5269

• MHD Rami Al Shweiki, Patrick Oeckl, Petra Steinacker, Peggy Barschke, Cornelia Dorner-Ciossek, Bastian Hengerer et al. Proteomic analysis reveals a biosignature of decreased synaptic protein in cerebrospinal fluid of major depressive disorder Translational Psychiatry 2020-05-12 [PMID: 32398672]
• MHD Rami Al Shweiki, Patrick Oeckl, Petra Steinacker, Peggy Barschke, Cornelia Dorner-Ciossek, Bastian Hengerer et al. Proteomic analysis reveals a biosignature of decreased synaptic protein in cerebrospinal fluid of major depressive disorder Translational Psychiatry 2020-05-12 [PMID: 32398672] (Western Blot, Human)

Bioinformatics

• Gene Symbol: NRXN3
• Uniprot:
  • NP_620426(Human)