HAF016). A specific band was detected for Neurexin 1 beta /NXRN1b at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat Neurexin 1 beta /NXRN1b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4524) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurexin 1 beta /NXRN1b at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4524-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94557-0.02ml | |||
![Western Blot: NRXN1 Antibody [NBP1-00219] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with (1/3000) in lane C. Mock-transfected HEK293 probed with NBP1-00219 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NRXN1-Antibody-Western-Blot-NBP1-00219-img0004.jpg "Western Blot: NRXN1 Antibody [NBP1-00219] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with (1/3000) in lane C. Mock-transfected HEK293 probed with NBP1-00219 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.")
![Flow Cytometry: NRXN1 Antibody [NBP1-00219] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/NRXN1-Antibody-Flow-Cytometry-NBP1-00219-img0005.jpg)
![ELISA: NRXN1 Antibody (4F7) [H00009378-M02] - Detection limit for recombinant GST tagged NRXN1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NRXN1-Antibody-4F7-Sandwich-ELISA-H00009378-M02-img0001.jpg "ELISA: NRXN1 Antibody (4F7) [H00009378-M02] - Detection limit for recombinant GST tagged NRXN1 is 0.1 ng/ml as a capture antibody.")
![Western Blot: Neurexin 1 beta/NXRN1b Antibody - BSA Free [NBP3-47305] - SH-SY5Y cells were subjected to SDS PAGE followed by western blot with (Neurexin 1 beta/NXRN1b antibody) at dilution of 1:500](http://images.novusbio.com/fullsize/nbp3-47305_rabbit-neurexin-1-beta-nxrn1b-pab-bsa-free-28420251221127.jpg "Western Blot: Neurexin 1 beta/NXRN1b Antibody - BSA Free [NBP3-47305] - SH-SY5Y cells were subjected to SDS PAGE followed by western blot with (Neurexin 1 beta/NXRN1b antibody) at dilution of 1:500")
![SDS-Page: Recombinant Human NRXN1 Protein [H00009378-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00009378-Q01-1.jpg "SDS-Page: Recombinant Human NRXN1 Protein [H00009378-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: NRXN1 Antibody [NBP2-94557] - Analysis of extracts of various cell lines, using NRXN1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/NRXN1-Antibody-Western-Blot-NBP2-94557-img0001.jpg "Western Blot: NRXN1 Antibody [NBP2-94557] - Analysis of extracts of various cell lines, using NRXN1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: NRXN1 Overexpression Lysate (Adult Normal) [NBL1-13805] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRXN1.](http://images.novusbio.com/fullsize/NRXN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13805-img0002.jpg "Western Blot: NRXN1 Overexpression Lysate (Adult Normal) [NBL1-13805] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRXN1.")
![Western Blot: NRXN1 Overexpression Lysate (Adult Normal) [NBP2-09575] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRXN1.](http://images.novusbio.com/fullsize/NRXN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09575-img0001.jpg "Western Blot: NRXN1 Overexpression Lysate (Adult Normal) [NBP2-09575] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRXN1.")
![Western Blot: NRXN1 Antibody [NBP2-87943] - Host: Rabbit. Target Name: NRXN1. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/NRXN1-Antibody-Western-Blot-NBP2-87943-img0001.jpg "Western Blot: NRXN1 Antibody [NBP2-87943] - Host: Rabbit. Target Name: NRXN1. Sample Tissue: Human 786-0 Whole Cell. Antibody Dilution: 1.0ug/ml")
![Western Blot: NRXN1 Antibody [NBP2-87932] - Host: Rabbit. Target Name: NRXN1. Sample Tissue: Human PRMI 8226 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/NRXN1-Antibody-Western-Blot-NBP2-87932-img0001.jpg "Western Blot: NRXN1 Antibody [NBP2-87932] - Host: Rabbit. Target Name: NRXN1. Sample Tissue: Human PRMI 8226 Whole Cell lysates. Antibody Dilution: 1ug/ml")
