Human Neuregulin-3/NRG3 ELISA Kit (Colorimetric)

ELISA: Human Neuregulin-3/NRG3 ELISA Kit (Colorimetric) [NBP2-76761] - Samples were spiked with high concentrations of Human Neuregulin-3/NRG3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Neuregulin-3/NRG3 ELISA Kit (Colorimetric) [NBP2-76761] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.156 - 10ng/mL
• Sensitivity: 0.094ng/mL

Human Neuregulin-3/NRG3 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.156 - 10ng/mL
• Sensitivity: 0.094ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.41
• Intra-Assay: CV% < 4.51
• Spike Recovery: 87-105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NRG3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Neuregulin-3/NRG3 ELISA Kit (Colorimetric)

• HRG3
• neuregulin 3 variant 1
• neuregulin 3 variant 10
• neuregulin 3 variant 11
• neuregulin 3 variant 12
• neuregulin 3 variant 13
• neuregulin 3 variant 14
• neuregulin 3 variant 3
• neuregulin 3 variant 4
• neuregulin 3 variant 5
• neuregulin 3 variant 6
• neuregulin 3 variant 7
• neuregulin 3 variant 8
• neuregulin 3 variant 9
• neuregulin 3
• Neuregulin3
• Neuregulin-3
• neuregulin-3-like polypeptide
• NRG3
• pro-neuregulin-3, membrane-bound isoform
• pro-NRG3

Background

NRG3, which belongs to the neuregulin family, is a direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. NRG3 does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. The protein exists as an type I membrane protein and as a proteolytically released soluble growth factor form. The membrane-bound form does not appear to be active. NRG3 is highly expressed in most regions of the brain with the exception of corpus callosum, and is expressed at lower level in testis. It is not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes. The NRG3 cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing may involve initial intracellular domain dimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Neuregulin-3/NRG3 ELISA Kit (NBP2-76761). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NRG3