Neuregulin-3/NRG3 Antibody

Western Blot: Neuregulin-3/NRG3 Antibody [NB100-61670] - Analysis of Neuregulin 3 in Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Neuregulin-3/NRG3 Antibody Summary

• Immunogen: Peptide with sequence C-RNEIQRDSALTK corresponding to C-Terminus according to NP_001010848.2.
• Epitope: RNEIQRDSALTK
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRG3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in human brain (cerebellum) lysates and in mouse and rat brain lysates (calculated MW of 75.0 kDa band according to NP_001010848.2). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neuregulin-3/NRG3 Antibody

• HRG3
• neuregulin 3 variant 1
• neuregulin 3 variant 10
• neuregulin 3 variant 11
• neuregulin 3 variant 12
• neuregulin 3 variant 13
• neuregulin 3 variant 14
• neuregulin 3 variant 3
• neuregulin 3 variant 4
• neuregulin 3 variant 5
• neuregulin 3 variant 6
• neuregulin 3 variant 7
• neuregulin 3 variant 8
• neuregulin 3 variant 9
• neuregulin 3
• Neuregulin3
• Neuregulin-3
• neuregulin-3-like polypeptide
• NRG3
• pro-neuregulin-3, membrane-bound isoform
• pro-NRG3

Background

NRG3, which belongs to the neuregulin family, is a direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. NRG3 does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. The protein exists as an type I membrane protein and as a proteolytically released soluble growth factor form. The membrane-bound form does not appear to be active. NRG3 is highly expressed in most regions of the brain with the exception of corpus callosum, and is expressed at lower level in testis. It is not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes. The NRG3 cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing may involve initial intracellular domain dimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuregulin-3/NRG3 Antibody (NB100-61670)(1)

Publication using NB100-61670

• Hijazi MM, Young PE, Dougherty MK et al. NRG-3 in human breast cancers: activation of multiple erbB family proteins. Int J Oncol 1998-11-01 [PMID: 9772300]

Bioinformatics

• Gene Symbol: NRG3
• Entrez:
  • 10718(Human)
  • 18183(Mouse)
  • 498596(Rat)
• Uniprot:
  • NP_001010848.2(Human)