Recombinant Human Neuregulin-1 alpha/NRG1 alpha Protein

SDS-Page: Human Neuregulin-1 alpha/NRG1 alpha Protein [NBP2-35093]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human Neuregulin-1 alpha/NRG1 alpha Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 65 amino acids corresponding to Neuregulin-1 alpha/NRG1 alpha Source: E. coli

  Uniprot ID: Q02297

  Amino Acid Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCQPGF TGARCTENVP MKVQNQEKAE ELYQK

• Details of Functionality: Neuregulin-1 alpha/NRG1 alpha protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 x 10^4 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NRG1
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Neuregulin-1 alpha/NRG1 alpha as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 7.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in 20 mM PB, pH 6.0, 150 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human Neuregulin-1 alpha/NRG1 alpha Protein

• ARIA
• GGF2
• HGL
• HRG
• HRG1
• MST131
• MSTP131
• neuregulin 1
• Neuregulin1 alpha
• Neuregulin-1 alpha
• pro-neuregulin-1, membrane-bound isoform
• pro-NRG1
• sensory and motor neuron derived factor
• SMDF

Background

Neuregulin 1 (NRG1) is in humans encoded by the NRG1 gene. It is one of four proteins belonging to the neuregulin family that act on the EGFR family of receptor. Neuregulin 1 is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. All NRG1 isoforms contain an EGF like domain (alpha or beta splice variant that differ in their C terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. NRG1 is necessary for cardiac development, structural maintenance, and functional integrity of the heart. NRG1 and its receptor family ErbB can play a beneficial role in the treatment of chronic heart failure (CHF) by promoting survival of cardiac myocytes, improving sarcomeric structure, balancing Ca2+ homeostasis, and enhancing pumping function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NRG1
• Uniprot:
  • Q02297()
  • Q02297()
  • Q02297()