Neudesin Antibody (737130)

Images

 
Western blot shows lysates of MCF-7 human breast cancer cell line, IMR-32 human neuroblastoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Neudesin ...read more
Neudesin was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Neudesin Monoclonal Antibody (Catalog # MAB6714) at 15 µg/mL overnight at 4 °C. Before incubation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
737130
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Neudesin Antibody (737130) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Neudesin
Gly32-Phe172
Accession # Q9UMX5
Specificity
Detects human Neudesin in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NENF
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB6714 in the following applications:

  • ICC
    1 publication
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neudesin Antibody (737130)

  • Cell immortalization-related protein 2
  • CIR2
  • CIR2cell growth-inhibiting protein 47
  • NENF
  • Neudesin
  • neuron derived neurotrophic factor
  • Neuron-derived neurotrophic factor
  • SCIRP10
  • SCIRP10-related protein
  • Secreted protein of unknown function
  • Spinal cord injury related protein 10
  • SPUF protein
  • SPUF
  • SPUFneudesin

Background

Neudesin (Neuron-derived neurotrophic secreted protein; also CIR2 and SPUF) is a secreted, 20-21 kDa member of the MAPR (membrane-associated progesterone receptor) subfamily, cytochrome b5 family of molecules. It is expressed by CNS neuronal progenitors and neurons, plus preadipocytes in white adipose tissue. Regarding activity, it promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor. By contrast, it inhibits both astrocyte and adipocyte differentiation. Mature human Neudesin is 141 amino acids (aa) in length (aa 32-172). It contains one cytochrome b5-like heme-binding domain (aa 44-129) and an acetylated lysine residue at Lys136. The heme-binding domain does bind heme, and this accounts for 5-6 kDa of its circulating molecular weight. The presence of a heme moiety is necessary for activity. Mature human Neudesin shares 97% aa identity with mature mouse Neudesin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neudesin Antibody (MAB6714)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, IHC.


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(1)
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(1)
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Bioinformatics

Gene Symbol NENF
Uniprot