Neudesin Antibody (737130)

Western blot shows lysates of MCF-7 human breast cancer cell line, IMR-32 human neuroblastoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Neudesin Monoclonal Antibody (Catalog # MAB6714) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Neudesin at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neudesin was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Neudesin Monoclonal Antibody (Catalog # MAB6714) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 737130
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Neudesin Antibody (737130) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Neudesin
  Gly32-Phe172
  Accession # Q9UMX5
• Specificity: Detects human Neudesin in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NENF
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neudesin Antibody (737130)

• Cell immortalization-related protein 2
• CIR2
• CIR2cell growth-inhibiting protein 47
• NENF
• Neudesin
• neuron derived neurotrophic factor
• Neuron-derived neurotrophic factor
• SCIRP10
• SCIRP10-related protein
• Secreted protein of unknown function
• Spinal cord injury related protein 10
• SPUF protein
• SPUF
• SPUFneudesin

Background

Neudesin (Neuron-derived neurotrophic secreted protein; also CIR2 and SPUF) is a secreted, 20-21 kDa member of the MAPR (membrane-associated progesterone receptor) subfamily, cytochrome b5 family of molecules. It is expressed by CNS neuronal progenitors and neurons, plus preadipocytes in white adipose tissue. Regarding activity, it promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor. By contrast, it inhibits both astrocyte and adipocyte differentiation. Mature human Neudesin is 141 amino acids (aa) in length (aa 32-172). It contains one cytochrome b5-like heme-binding domain (aa 44-129) and an acetylated lysine residue at Lys136. The heme-binding domain does bind heme, and this accounts for 5-6 kDa of its circulating molecular weight. The presence of a heme moiety is necessary for activity. Mature human Neudesin shares 97% aa identity with mature mouse Neudesin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neudesin Antibody (MAB6714)(1)

Publication using MAB6714

• Kami?ska, J;Koper-Lenkiewicz, OM;Ponikwicka-Tyszko, D;Lebiedzi?ska, W;Palak, E;Sztachelska, M;Bernaczyk, P;Dorf, J;Guzi?ska-Ustymowicz, K;Zar?ba, K;Wo?czy?ski, S;Rahman, NA;Dymicka-Piekarska, V; New Insights on the Progesterone (P4) and PGRMC1/NENF Complex Interactions in Colorectal Cancer Progression Cancers 2023-10-20 [PMID: 37894441] (ICC, IHC, Human)

Bioinformatics

• Gene Symbol: NENF
• Uniprot:
  • Q9UMX5(Human)