NEK7 Overexpression Lysate

Images

 
Western Blot: NEK7 Overexpression Lysate (Adult Normal) [NBL1-13587] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NEK7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NEK7 Overexpression Lysate Summary

Description

NEK7 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC211857

Accession#: NM_133494

Protein Tag: C-MYC/DDK
Gene
NEK7

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
34.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NEK7 Overexpression Lysate

  • EC 2.7.11.1
  • Never in mitosis A-related kinase 7
  • NIMA (never in mitosis gene a)-related kinase 7
  • NimA-related protein kinase 7
  • serine/threonine-protein kinase Nek7

Background

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47865
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00091754-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
H00004751-M11
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
NBP1-82884
Species: Hu, Mu
Applications: IHC, IHC-P
NBP1-48291
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
H00284086-P01
Species: Hu
Applications: ELISA, AP, PA, WB
H00121441-M05
Species: Bv, Hu, Mu, Po, Rt, Sh
Applications: ELISA, IB, ICC/IF, IHC, IHC-P, WB
NB100-1595
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-87799
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB500-181
Species: Dr, Hu, Ma, Mu, Pl, Po, Rt
Applications: IB, ICC/IF, IP, WB
NBP2-67796
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB100-56537
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB
NBP2-13650
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-67674
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AF3064
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
NBP1-87195
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P
H00079858-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP2-76884
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
H00152110-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, S-ELISA, WB
NBL1-13587
Species: Hu
Applications: WB

Publications for NEK7 Lysate (NBL1-13587) (0)

There are no publications for NEK7 Lysate (NBL1-13587).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NEK7 Lysate (NBL1-13587) (0)

There are no reviews for NEK7 Lysate (NBL1-13587). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NEK7 Lysate (NBL1-13587) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NEK7 Products

Bioinformatics Tool for NEK7 Lysate (NBL1-13587)

Discover related pathways, diseases and genes to NEK7 Lysate (NBL1-13587). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NEK7 Lysate (NBL1-13587)

Discover more about diseases related to NEK7 Lysate (NBL1-13587).
 

Pathways for NEK7 Lysate (NBL1-13587)

View related products by pathway.

PTMs for NEK7 Lysate (NBL1-13587)

Learn more about PTMs related to NEK7 Lysate (NBL1-13587).
 

Research Areas for NEK7 Lysate (NBL1-13587)

Find related products by research area.

Blogs on NEK7

There are no specific blogs for NEK7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Eg5 Antibody
NB500-181

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NEK7 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol NEK7