NEK3 Antibody (2F8) - Azide and BSA Free

Western Blot: NEK3 Antibody (2F8) [H00004752-M01] - Analysis of NEK3 expression in transfected 293T cell line by NEK3 monoclonal antibody (M01), clone 2F8.Lane 1: NEK3 transfected lysate(57.7 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: NEK3 Antibody (2F8) [H00004752-M01] - Analysis of monoclonal antibody to NEK3 on HeLa cell. Antibody concentration 10 ug/ml.

ELISA: NEK3 Antibody (2F8) [H00004752-M01] - Detection limit for recombinant GST tagged NEK3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NEK3 Antibody (2F8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NEK3 (AAH19916, 406 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QWLKETPDTLLNILKNADLSLAFQTYTIYRPGSEGFLKGPLSEETEASDSVDGGHDSVILDPERLEPGLDEEDTDFEEEDDNPDWVSELKKRAGWQGLCDR
• Specificity: NEK3 - NIMA (never in mitosis gene a)-related kinase 3
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEK3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NEK3 Antibody (2F8) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• glycogen synthase A kinase
• HSPK 36
• HSPK36
• hydroxyalkyl-protein kinase
• MGC29949
• Never in mitosis A-related kinase 3
• NIMA (never in mitosis gene a)-related kinase 3
• NimA-related protein kinase 3
• phosphorylase B kinase kinase
• serine/threonine-protein kinase Nek3

Background

In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK3
• Entrez:
  • 4752(Human)
• OMIM:
  • 604044(Human)
• Uniprot:
  • AAH19916(Human)