Recombinant Human NEK2 Protein

Product Discontinued

Recombinant Human NEK2 Protein Summary

• Description: Recombinant protein for Human NEK2
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(P51955)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: NEK2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 183 nmol phosphate incorporated into MBP per minute per mg protein at 300C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human NEK2 Protein

• EC 2.7.11
• EC 2.7.11.1
• HSPK 21
• HsPK21
• NEK2
• NEK2AHsPK21
• Never in mitosis A-related kinase 2
• NIMA (never in mitosis gene a)-related kinase 2
• NimA-like protein kinase 1
• NimA-related protein kinase 2
• NLK1
• NLK1serine/threonine-protein kinase Nek2

Background

Nek 2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2 (1). These observations demonstrate that Nek2 resembles Aspergillus NIMA, not only in its catalytic domain, but also in its cell cycle-dependent expression. Recombinant Nek2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human Nek2 and fungal NIMA phosphorylate a similar, albeit not identical, set of proteins and synthetic peptides, and Beta -casein is a suitable substrate for assaying Nek2 in vitro (2). Nek2 is shown to be expressed most abundantly in the testis of the adult tissues examined (3). Its expression in the testis is restricted to the germ cells, with highest levels detected in spermatocytes at pachytene and diplotene stages. Immunohistochemical analysis revealed that Nek2 localized to nuclei, exhibiting a non-uniform distribution within the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NEK2