NEK2 Antibody

Immunohistochemistry-Paraffin: NEK2 Antibody [NBP1-02793] - Staining of human colon tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Immunohistochemistry-Paraffin: NEK2 Antibody [NBP1-02793] - Staining of human testis tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Product Discontinued

NEK2 Antibody Summary

• Immunogen: Synthetic peptide corresponding to an internal region near aa 275-300 of human NEK2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK2 Antibody

• EC 2.7.11
• EC 2.7.11.1
• HSPK 21
• HsPK21
• NEK2
• NEK2AHsPK21
• Never in mitosis A-related kinase 2
• NIMA (never in mitosis gene a)-related kinase 2
• NimA-like protein kinase 1
• NimA-related protein kinase 2
• NLK1
• NLK1serine/threonine-protein kinase Nek2

Background

NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK2
• Entrez:
  • 4751(Human)
• Uniprot:
  • P51955(Human)