NEK2 Antibody

Western Blot: NEK2 Antibody [H00004751-B01P] - Analysis of NEK2 expression in rat brain.

Immunocytochemistry/Immunofluorescence: NEK2 Antibody [H00004751-B01P] - Analysis of purified antibody to NEK2 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: NEK2 Antibody [H00004751-B01P] - Analysis of NEK2 expression in transfected 293T cell line by NEK2 polyclonal antibody. Lane 1: NEK2 transfected lysate(48.95 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

NEK2 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: NEK2 (NP_002488.1, 1 a.a. - 445 a.a.) full-length human protein. MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR
• Specificity: NEK2 - NIMA (never in mitosis gene a)-related kinase 2,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: NEK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NEK2 Antibody

• EC 2.7.11
• EC 2.7.11.1
• HSPK 21
• HsPK21
• NEK2
• NEK2AHsPK21
• Never in mitosis A-related kinase 2
• NIMA (never in mitosis gene a)-related kinase 2
• NimA-like protein kinase 1
• NimA-related protein kinase 2
• NLK1
• NLK1serine/threonine-protein kinase Nek2

Background

Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK2
• Entrez:
  • 4751(Human)
• Uniprot:
  • NP_002488.1(Human)