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NEDP1/SENP8 Antibody (2E1)

Images

 
Western Blot: NEDP1/SENP8 Antibody (2E1) [H00123228-M06] - Analysis of SENP8 expression in transfected 293T cell line by SENP8 monoclonal antibody (M06), clone 2E1. Lane 1: SENP8 transfected lysatE (24.1 KDa). Lane 2: ...read more
Sandwich ELISA: NEDP1/SENP8 Antibody (2E1) [H00123228-M06] - Detection limit for recombinant GST tagged SENP8 is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NEDP1/SENP8 Antibody (2E1) Summary

Immunogen
SENP8 (NP_660205, 113 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NSFFHYDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLIATLAKK
Specificity
SENP8 - SUMO/sentrin specific peptidase family member 8 (2E1)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SENP8
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NEDP1/SENP8 Antibody (2E1)

  • DEN1
  • DEN1deneddylase-1
  • deneddylase 1
  • Deneddylase-1
  • EC 3.4.22.-
  • HsT17512
  • NEDD8-specific protease 1
  • NEDP1
  • NEDP1NEDD8 specific-protease cysteine 2
  • Protease, cysteine 2
  • protease, cysteine, 2 (NEDD8 specific)
  • PRSC2
  • SENP8
  • Sentrin/SUMO-specific protease SENP8
  • sentrin-specific protease 8
  • SUMO sentrin specific protease family member 8
  • SUMO/sentrin specific peptidase family member 8
  • SUMO/sentrin specific protease family member 8

Background

NEDD8 (MIM 603171) is a ubiquitin-like protein that becomes conjugated to the cullin (see CUL1; MIM 603134) subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. NEDD8-specific deneddylases, such as NEDP1, or DEN1, are required to process the NEDD8 propeptide at a C-terminal diglycine motif and to remove NEDD8 from cullins (Gan-Erdene et al., 2003 [PubMed 12759363]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SENP8
Entrez
Uniprot