NEDD9/CASL/HEF1 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

NEDD9/CASL/HEF1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human NEDD9
  Lys153-Asn366
  Accession # Q14511
• Specificity: Detects endogenous human NEDD9 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NEDD9/CASL/HEF1 Antibody [Alexa Fluor® 647]

• Cas scaffolding protein family member 2
• CAS2
• CASL
• CAS-LCASS2cas-like docking
• CASLdJ49G10.2
• CRK-associated substrate-related protein
• dJ49G10.2 (Enhancer of Filamentation 1 (HEF1))
• dJ761I2.1 (enhancer of filamentation (HEF1))
• dJ761I2.1
• enhancer of filamentation 1
• HEF1
• HEF1Crk-associated substrate related
• NEDD9
• NEDD-9
• Neural precursor cell expressed developmentally down-regulated protein 9
• neural precursor cell expressed, developmentally down-regulated 9
• p105
• Renal carcinoma antigen NY-REN-12

Background

NEDD9 (also HEF1 and Cas-L) is a 93 kDa (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin beta 1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834 amino acids (aa) in length and contains one SH3 domain (aa 3‑65), a Tyr-rich SH2-binding region (aa 90‑350), one Ser-rich four alpha -helix bundle protein interaction site (aa 350‑650), and an HLH motif that mediates Cas protein dimerization (aa 710‑760). NEDD9 usually runs at 105 or 115 kDa in SDS‑PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55 kDa fragment. One isoform shows a deletion of aa 155‑188, while two others show 20 and seven aa substitutions for aa 155‑834 and 1‑153, respectively. Over aa 153‑366, human and mouse NEDD9 share 85% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.