Recombinant Human NEDD8 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Concentration
1 mg/ml

Order Details

Recombinant Human NEDD8 Protein Summary

Description
Recombinant protein for Human NEDD8-Aminomethylcoumarin
Amino Acid Sequence:(Q15843)
Protein/Peptide Type
Recombinant Protein
Gene
NEDD8
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Application Notes
1. As a substrate for NEDDylating enzyme activity assays
2. Identification/confirmation of enzyme deVEDDylation activity
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL (ubiquitin-like)-AMC substrates
Substrate concentration: 10 nM - 1.0 uM
Enzyme Concentration: 10 pM - 100 nM
Fluorescence of AMC released by the enzymes can be monitored using a fluorometer (Ex: 380 nm; Em: 460 nm).
Theoretical MW
8.735 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
A solution in DMSO
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human NEDD8 Protein

  • FLJ43224
  • MGC104393
  • MGC125896
  • MGC125897
  • NEDD8
  • NEDD-8
  • Neddylin
  • Neural precursor cell expressed developmentally down-regulated protein 8
  • neural precursor cell expressed, developmentally down-regulated 8
  • Rub1
  • Ubiquitin-like protein Nedd8

Background

Prepared by the C-terminal derivatisation of NEDD8 with 7-amino-4-methylcoumarin. NEDD8-AMC acts as a useful and sensitive fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Blogs on NEDD8.

PINK1: All work and no fun
The protein PINK1 is a mitochondrial-located serine/threonine kinase (PTK) that maintains organelle function and integrity. It not only protects organelles from cellular stress, but it also uses the selective auto-phagocytosis process for cleaning and...  Read full blog post.

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Bioinformatics

Gene Symbol NEDD8
Entrez