Recombinant Human NEDD8 Protein

Product Discontinued

Recombinant Human NEDD8 Protein Summary

• Description: Recombinant protein for Human NEDD8-Aminomethylcoumarin
  
  

  Source:Synthetic
  Amino Acid Sequence:(Q15843)

• Source: Synthetic
• Protein/Peptide Type: Recombinant Protein
• Gene: NEDD8
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: 1. As a substrate for NEDDylating enzyme activity assays
  2. Identification/confirmation of enzyme deVEDDylation activity
  3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL (ubiquitin-like)-AMC substrates
  Substrate concentration: 10 nM - 1.0 uM
  Enzyme Concentration: 10 pM - 100 nM
  Fluorescence of AMC released by the enzymes can be monitored using a fluorometer (Ex: 380 nm; Em: 460 nm).
• Theoretical MW: 8.735 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: A solution in DMSO
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human NEDD8 Protein

• FLJ43224
• MGC104393
• MGC125896
• MGC125897
• NEDD8
• NEDD-8
• Neddylin
• Neural precursor cell expressed developmentally down-regulated protein 8
• neural precursor cell expressed, developmentally down-regulated 8
• Rub1
• Ubiquitin-like protein Nedd8

Background

Prepared by the C-terminal derivatisation of NEDD8 with 7-amino-4-methylcoumarin. NEDD8-AMC acts as a useful and sensitive fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NEDD8