NEDD8 Antibody (JF69-09)

Immunocytochemistry/Immunofluorescence: NEDD8 Antibody (JF69-09) [NBP2-66970] - Staining NEDD8 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: NEDD8 Antibody (JF69-09) [NBP2-66970] - Analysis of paraffin-embedded human testis tissue with Rabbit anti-NEDD8 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: NEDD8 Antibody (JF69-09) [NBP2-66970] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-NEDD8 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NEDD8 Antibody (JF69-09) [NBP2-66970] - Analysis of paraffin-embedded human lung cancer tissue using anti-NEDD8 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NEDD8 Antibody (JF69-09) [NBP2-66970] - Analysis of paraffin-embedded rat bladder tissue with Rabbit anti-NEDD8 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

NEDD8 Antibody (JF69-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human NEDD8 aa 12-57 / 81. (SwissProt: Q15843 Human; SwissProt: P29595 Mouse; SwissProt: Q71UE8 Rat)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NEDD8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NEDD8 Antibody (JF69-09)

• FLJ43224
• MGC104393
• MGC125896
• MGC125897
• NEDD8
• NEDD-8
• Neddylin
• Neural precursor cell expressed developmentally down-regulated protein 8
• neural precursor cell expressed, developmentally down-regulated 8
• Rub1
• Ubiquitin-like protein Nedd8

Background

NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEDD8