Nectin-4/PVRL4 Antibody [Unconjugated]

Images

 
Nectin‑4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Nectin-4/PVRL4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nectin-4
Gly27-Val351
Accession # Q96NY8
Specificity
Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross‑reactivity with recombinant mouse Nectin-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PVRL4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2659 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-4/PVRL4 Antibody [Unconjugated]

  • EDSS1
  • Ig superfamily receptor LNIR
  • LNIR
  • LNIRnectin 4
  • Nectin4
  • Nectin-4
  • poliovirus receptor-related 4
  • poliovirus receptor-related protein 4
  • PRR4
  • PRR4DKFZp686K05193
  • PVRL4

Background

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF4330
Species: Mu
Applications: CyTOF-ready, Flow, WB
AF2880
Species: Hu, Mu
Applications: WB
MAB2005
Species: Hu
Applications: CyTOF-ready, Flow, WB
MAB25301
Species: Hu
Applications: CyTOF-ready, Flow, ICC, WB
AF7829
Species: Hu
Applications: ICC, IHC, WB
NBP2-34594
Species: Hu, Pm
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P
H00005670-Q01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
AF4478
Species: Hu
Applications: IHC, WB
AF3064
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
NB300-186
Species: Hu, Rt
Applications: ICC/IF, Simple Western, WB
AF5415
Species: Hu
Applications: ICC, IHC, Simple Western, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF761
Species: Mu
Applications: AdBlk, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, Simple Western, WB
H00005555-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
AF2605
Species: Hu
Applications: ICC, IHC, Simple Western, WB
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF2659
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready

Publications for Nectin-4/PVRL4 Antibody (AF2659)(11)

We have publications tested in 5 confirmed species: Human, Mouse, Canine, Hamster, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 5 applications: Functional Assay, ICC, IHC, IHC-P, Neutralization.


Filter By Application
Functional Assay
(1)
ICC
(2)
IHC
(4)
IHC-P
(3)
Neutralization
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
Canine
(3)
Hamster
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF2659 Applications Species
K Iizuka, K Shoji, T Fujiyuki, K Moritoh, K Tamura, A Yoshida, H Sato, M Yoneda, K Asano, C Kai Antitumor activity of an oncolytic measles virus against canine urinary bladder transitional cell carcinoma cells Research in Veterinary Science, 2020;0(0):. 2020 [PMID: 33183781] (IHC, Canine) IHC Canine
H Deng, H Shi, L Chen, Y Zhou, J Jiang Over-expression of Nectin-4 promotes progression of esophageal cancer and correlates with poor prognosis of the patients Cancer Cell Int., 2019;19(0):106. 2019 [PMID: 31043861] (IHC, Human) IHC Human
W Pratakpiri, AP Ping Teh, A Radtanakat, N Pirarat, N Thi Lan, M Takeda, S Techangams, R Yamaguchi Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogs Sci Rep, 2017;7(1):349. 2017 [PMID: 28336928] (IHC-P, Canine) IHC-P Canine
M Kitayama, K Mizutani, M Maruoka, K Mandai, S Sakakibara, Y Ueda, T Komori, Y Shimono, Y Takai A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development J. Biol. Chem, 2016;291(11):5817-31. 2016 [PMID: 26757815] (ICC, Mouse) ICC Mouse
Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer. J Exp Clin Cancer Res, 2015;34(0):30. 2015 [PMID: 25888293] (IHC-P, Human) IHC-P Human
Alves L, Khosravi M, Avila M, Ader-Ebert N, Bringolf F, Zurbriggen A, Vandevelde M, Plattet P SLAM- and Nectin-4-Independent Noncytolytic Spread of Canine Distemper Virus in Astrocytes. J Virol, 2015;89(10):5724-33. 2015 [PMID: 25787275] (IHC, Canine) IHC Canine
Galanis E, Atherton P, Maurer M, Knutson K, Dowdy S, Cliby W, Haluska P, Long H, Oberg A, Aderca I, Block M, Bakkum-Gamez J, Federspiel M, Russell S, Kalli K, Keeney G, Peng K, Hartmann L Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer. Cancer Res, 2015;75(1):22-30. 2015 [PMID: 25398436] (IHC, Human) IHC Human
Fortugno P, Josselin E, Tsiakas K, Agolini E, Cestra G, Teson M, Santer R, Castiglia D, Novelli G, Dallapiccola B, Kurth I, Lopez M, Zambruno G, Brancati F Nectin-4 mutations causing ectodermal dysplasia with syndactyly perturb the rac1 pathway and the kinetics of adherens junction formation. J Invest Dermatol, 2014;134(8):2146-53. 2014 [PMID: 24577405] (ICC, Human) ICC Human
Pavlova N, Pallasch C, Elia A, Braun C, Westbrook T, Hemann M, Elledge S A role for PVRL4-driven cell-cell interactions in tumorigenesis. Elife, 2013;2(0):e00358. 2013 [PMID: 23682311] (Functional Assay, Human) Functional Assay Human
Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008. J Virol, 2013;87(2):1105-14. 2013 [PMID: 23135729] (IHC-P, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) IHC-P Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Show All 11 Publications.

Reviews for Nectin-4/PVRL4 Antibody (AF2659) (0)

There are no reviews for Nectin-4/PVRL4 Antibody (AF2659). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Nectin-4/PVRL4 Antibody (AF2659) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2659

Additional Nectin-4/PVRL4 Products

Bioinformatics Tool for Nectin-4/PVRL4 Antibody (AF2659)

Discover related pathways, diseases and genes to Nectin-4/PVRL4 Antibody (AF2659). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nectin-4/PVRL4 Antibody (AF2659)

Discover more about diseases related to Nectin-4/PVRL4 Antibody (AF2659).
 

Pathways for Nectin-4/PVRL4 Antibody (AF2659)

View related products by pathway.

PTMs for Nectin-4/PVRL4 Antibody (AF2659)

Learn more about PTMs related to Nectin-4/PVRL4 Antibody (AF2659).

Blogs on Nectin-4/PVRL4

There are no specific blogs for Nectin-4/PVRL4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Nectin-4/PVRL4 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol PVRL4
Uniprot