Nectin-4/PVRL4 Antibody (356704)

• Reactivity: Mu
• Applications: WB
• Clone: 356704
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Nectin-4/PVRL4 Antibody (356704) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Nectin-4
  Tyr28-Ile349
  Accession # Q8R007
• Specificity: Detects mouse Nectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human Nectin‑4, 10% cross-reactivity with recombinant mouse (rm) Nectin-3, and no cross-reactivity with rmNectin-1 or rmNectin-2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PVRL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-4/PVRL4 Antibody (356704)

• EDSS1
• Ig superfamily receptor LNIR
• LNIR
• LNIRnectin 4
• Nectin4
• Nectin-4
• poliovirus receptor-related 4
• poliovirus receptor-related protein 4
• PRR4
• PRR4DKFZp686K05193
• PVRL4

Background

Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma. A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of mouse Nectin-4 shares 90% and 95% amino acid sequence homology with the corresponding regions of human and rat Nectin-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nectin-4/PVRL4 Antibody (MAB3116)(2)

Publications using MAB3116

• Adi Reches, Yael Ophir, Natan Stein, Inbal Kol, Batya Isaacson, Yoav Charpak Amikam et al. Nectin4 is a novel TIGIT ligand which combines checkpoint inhibition and tumor specificity Journal for ImmunoTherapy of Cancer 2020-06-04 [PMID: 32503945]
• Uchida H, Chan J, Goins WF A double mutation in glycoprotein gB compensates for ineffective gD-dependent initiation of herpes simplex virus type 1 infection. J. Virol., 2010-09-22;84(23):12200-9. 2010-09-22 [PMID: 20861246] (Bioassay, Hamster)

Bioinformatics

• Gene Symbol: PVRL4
• Uniprot:
  • Q8R007()