Nectin-3/PVRL3 Antibody (CL15364) - Azide and BSA Free

Analysis in human testis and tonsil tissues using NBP3-44180 antibody. Corresponding Nectin-3/PVRL3 RNA-seq data are presented for the same tissues.

Staining of human duodenum shows moderate to strong membranous positivity in glandular cells.

Staining of human pancreas shows moderate membranous positivity in the islets of Langerhans.

Analysis in human cerebral cortex tissue.

Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts.

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Staining of mouse brain shows moderate positivity in neuronal processes in the CA3 area of the hippocampus.

Staining of rat brain shows moderate positivity in neuronal processes in the CA3 area of the hippocampus.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL15364
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Nectin-3/PVRL3 Antibody (CL15364) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q9NQS3, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NECTIN3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Nectin-3/PVRL3 Antibody (CL15364) - Azide and BSA Free

• CD113 antigen
• CD113
• CDw113FLJ90624
• DKFZp566B0846
• nectin 3
• Nectin3
• Nectin-3
• poliovirus receptor-related 3
• poliovirus receptor-related protein 3
• PPR3
• PRR3CD113
• PVRL3
• PVRR3

Background

Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NECTIN3