NDUFA7 Antibody - BSA Free

Western Blot: NDUFA7 Antibody [NBP3-03240] - Analysis of extracts of various cell lines, using NDUFA7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Western Blot: NDUFA7 Antibody [NBP3-03240] - Analysis of extracts from normal (control) and NDUFA7 knockout (KO) 293T cells, using NDUFA7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDUFA7 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit NDUFA7 Antibody - BSA Free (NBP3-03240) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2). MASATRLIQRLRNWASGHDLQGKLQLRYQEISKRTQPPPKLPVGPSHKLSNNYYCTRDGRRESVPPSIIMSSQKALVSGKPAESSAVAATEKKAVTPAPPIKRWELSSDQPYL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFA7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NDUFA7 Antibody - BSA Free

• B14.5a
• CI-B14.5a
• complex I B14.5a subunit
• Complex I-B14.5a
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (14.5kD, B14.5a)
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa
• NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
• NADH-ubiquinone oxidoreductase subunit B14.5a

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFA7