NCK2 Antibody

Product Discontinued

NCK2 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 341-390 of Human Nck-2.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
• Application Notes: Immunohistochemistry (IHC) analyzes of NCK2 (I364) pAb in paraffin-embedded human colon carcinoma tissue.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NCK2 Overexpression Lysate

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCK2 Antibody

• GRB4
• NCK adaptor protein 2cytoplasmic protein NCK2
• Nck-2
• NCKbeta
• noncatalytic region of tyrosine kinase, beta
• SH2/SH3 adaptor protein NCK-beta

Background

The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKalpha) and NCK2 (NCKbeta), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of NCK1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2.The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCK2
• Entrez:
  • 8440(Human)