NCK1 Antibody

Western Blot: NCK1 Antibody [H00004690-B01P-50ug] - NCK1 MaxPab polyclonal antibody. Western Blot analysis of NCK1 expression in A-431.

Immunocytochemistry/Immunofluorescence: NCK1 Antibody [H00004690-B01P-50ug] - Purified MaxPab antibody to NCK1 on HeLa cell. [antibody concentration 20 ug/ml]

Western Blot: NCK1 Antibody [H00004690-B01P-50ug] - Analysis of NCK1 expression in transfected 293T cell line (H00004690-T01) by NCK1 MaxPab polyclonal antibody. Lane 1: NCK1 transfected lysate(41.47 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

NCK1 Antibody Summary

• Immunogen: NCK1 (NP_006144.1, 1 a.a. - 377 a.a.) full-length human protein. MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: NCK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NCK1 Antibody

• cytoplasmic protein NCK1
• MGC12668
• NCK adaptor protein 1melanoma NCK protein
• NCK1
• nck-1
• NCKalpha
• NCKNCK tyrosine kinase
• non-catalytic region of tyrosine kinase
• SH2/SH3 adaptor protein NCK-alpha

Background

Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCK1
• Entrez:
  • 4690(Human)
• OMIM:
  • 600508(Human)
• Uniprot:
  • NP_006144.1(Human)