NBR1 Antibody

Images

 
Western Blot: NBR1 Antibody [H00004077-B01P] - Analysis of NBR1 expression in transfected 293T cell line by NBR1 polyclonal antibody. Lane 1: NBR1 transfected lysate(106.26 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Mouse

Order Details

NBR1 Antibody Summary

Immunogen
NBR1 (NP_005890.2, 1 a.a. ~ 966 a.a) full-length human protein.MEPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDLNTIQIKYLDEENEEVSINSQGEYEEALKMAVKQGNQLQMQVHEGHHVVDEAPPPVVGAKRLAARAGKKPLAHYSSLVRVLGSDMKTPEDPAVQSFPLVPCDTDQPQDKPPDWFTSYLETFREQVVNETVEKLEQKLHEKLVLQNPSLGSCPSEVSMPTSEETLFLPENQFSWHIACNNCQRRIVGVRYQCSLCPSYNICEDCEAGPYGHDTNHV
Specificity
NBR1 - neighbor of BRCA1 gene 1,
Clonality
Polyclonal
Host
Mouse
Gene
NBR1
Purity
Protein A purified
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Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 Review rated 5
using
H00004077-B01P in the following applications:

Publications
Read Publications using H00004077-B01P.

Reactivity Notes

Human.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NBR1 Antibody

  • Cell migration-inducing gene 19 protein
  • FLJ98272
  • KIAA0049CA125
  • M17S2FLJ55359,1A1-3B
  • Membrane component chromosome 17 surface marker 2,1A13B
  • membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigenCA125)
  • migration-inducing protein 19
  • Neighbor of BRCA1 gene 1 protein
  • neighbor of BRCA1 gene 1
  • next to BRCA1 gene 1 protein
  • Protein 1A1-3B

Background

The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled coil motif, which is present in many genes with transformation potential, but the function of this protein is unknown. This gene is located on a region of chromosome 17q21.1 that is in close proximity to tumor suppressor gene BRCA1. Three alternatively spliced variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NBR1 Antibody (H00004077-B01P)(2)

Reviews for NBR1 Antibody (H00004077-B01P) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00004077-B01P:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot NBR1 H00004077-B01P
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reviewed by:
Anonymous
WB Human 08/17/2009
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Summary

ApplicationWestern Blot
Sample TestedHuman HeLa cells, Sample Amount: 20 ug
SpeciesHuman
Lot08270WULz

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 30 minutes, Blocking Temp: 37 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/500, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse IgG, Secondary Ab Dilution Ratio: 1/5000

Details

Detection NotesMethod: ECL, Exposure Time: 1min, Pos.Ctrl: untreated cells, Neg.Ctrl: cells treated w/ siRNA against NBR1, Band: 125 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NBR1 Antibody (H00004077-B01P) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for NBR1 Antibody (H00004077-B01P)

Discover related pathways, diseases and genes to NBR1 Antibody (H00004077-B01P). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on NBR1.

Why LC3B Antibodies Make Ideal Autophagosomes Membrane Markers
The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants; LC3A, LC3B and LC3C (He et al., 2003). LC3B is a subunit of the MAP1A and MAP1B microtubule-binding proteins and plays a central role autophagosome...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol NBR1
Entrez
Uniprot

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