Napsin A Antibody [Unconjugated]

Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Napsin A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Napsin A was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human kidney, loaded at 0.2 mg/mL. A specific band was detected for Napsin A at approximately 45 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human Napsin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8489) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Napsin A Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Napsin A
  Thr25-Gly420
  Accession # O96009
• Specificity: Detects human Napsin A in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAPSA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-10 ug/mL
  • Simple Western 0.5 ug/mL
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Napsin A Antibody [Unconjugated]

• Asp 4
• ASP4
• Aspartyl protease 4
• EC 3.4.23
• EC 3.4.23.-
• EC 3.4.23.15
• EC 3.4.23.3
• EC 3.4.23.5
• KAP
• Kdap
• NAP1napsin-A
• NAPA
• NAPApronapsin A
• NAPSA
• napsin A aspartic peptidase
• Napsin A
• Napsin-1
• SNAPA
• TA01/TA02

Background

Novel Aspartic Proteinase of the Pepsin Family or Napsin A is an aspartic proteinase that belongs to the peptidase A1 family and plays a role in pneumocyte surfactant processing. It consists of a 24-residue signal peptide, a 40-amino acid propart, the mature enzyme of 336 amino acids, and a C-terminal extension of 18 residues. Highest levels of Napsin A have been detected in adult lung (type II pneumocytes), fetal lung, and kidney tissues. Napsin A is also expressed at lower levels in adult spleen and at very low levels in peripheral blood leukocytes. Human napsin A shares 72.6% sequence identity with the mouse homolog.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Napsin A Antibody (AF8489)(1)

Publication using AF8489

• R Gro beta , R Bauer, F Krüger, E Rücker-Bra, LR Olari, L Ständker, N Preising, AA Rodríguez, C Conzelmann, F Gerbl, D Sauter, F Kirchhoff, B Hagemann, J Ga?anin, T Weil, YB Ruiz-Blanc, E Sanchez-Ga, WG Forssmann, A Mankertz, S Santibanez, S Stenger, P Walther, S Wiese, B Spellerber, J Münch A Placenta Derived C-Terminal Fragment of &amp;beta-Hemoglobin With Combined Antibacterial and Antiviral Activity Front Microbiol, 2020-04-06;11(0):508. 2020-04-06 [PMID: 32328038] (Dot Blot, Human)

Bioinformatics

• Gene Symbol: NAPSA
• Uniprot:
  • O96009()