Napsin A Antibody (NAPSA/4400R)

Images

 
Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/4400R) [NBP3-07869] - IHC analysis of formalin-fixed, paraffin-embedded human lung adenocarcinoma. Napsin A antibody (NAPSA/4400R) at 2ug/ml in PBS for 30min RT. ...read more
Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/4400R) [NBP3-07869] - IHC analysis of formalin-fixed, paraffin-embedded human lung adenocarcinoma. Napsin A antibody (NAPSA/4400R) at 2ug/ml in PBS for 30min RT. ...read more
Protein Array: Napsin A Antibody (NAPSA/4400R) [NBP3-07869] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Napsin A-Monospecific Recombinant Rabbit Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P, PA
Clone
NAPSA/4400R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Napsin A Antibody (NAPSA/4400R) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment (aa50-150) of human Napsin A protein (exact sequence is proprietary) (Uniprot: O96009 )
Localization
Cytoplasmic
Marker
Lung Adenocarcinoma Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NAPSA
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Notes

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-08378)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Napsin A Antibody (NAPSA/4400R)

  • Asp 4
  • ASP4
  • Aspartyl protease 4
  • EC 3.4.23
  • EC 3.4.23.-
  • EC 3.4.23.15
  • EC 3.4.23.3
  • EC 3.4.23.5
  • KAP
  • Kdap
  • NAP1napsin-A
  • NAPA
  • NAPApronapsin A
  • NAPSA
  • napsin A aspartic peptidase
  • Napsin A
  • Napsin-1
  • SNAPA
  • TA01/TA02

Background

Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. Other AA family proteinases include Pepsin, cathepsin-D, cathepsin-E and rennin. Of these proteinases, napsin is most closely related to cathepsin-D, sharing 46% identity at the amino acid level. There are two closely related Napsins, napsin A and napsin B, with 85% overall identity at the amino acid level. There are three isoforms. Napsin-A is also termed napsin-1, or TA02, a Tumor Adenocarcinoma marker. Napsin A may be involved in processing of pneumocyte surfactant precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NAPSA
Uniprot