Recombinant Bacteria NAD Synthetase His Protein

• Reactivity: Ba
• Applications: Enzyme Activity

Recombinant Bacteria NAD Synthetase His Protein Summary

• Description: NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) fused at the N-terminus to a His-tag.
  
  Source: E. coli

  Uniprot ID: P0A5L6

• Details of Functionality: ~0.1 U/mg protein (Ammonium or glutamine as nitrogen source) One unit is defined as the amount of enzyme that synthesizes 1umol NAD per min
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NADSYN1
• Purity: >98%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

M. tuberculosis

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM TRIS-HCl, pH 7.8
• Preservative: No Preservative
• Purity: >98%, by SDS-PAGE

Alternate Names for Recombinant Bacteria NAD Synthetase His Protein

• EC 6.3.5.1
• FLJ10631
• FLJ36703
• FLJ40627
• glutamine-dependent NAD synthetase
• glutamine-dependent NAD(+) synthetase
• NAD synthetase 1
• NAD(+) synthase [glutamine-hydrolyzing]
• NAD(+) synthase
• NAD(+) synthetase

Background

Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase (EC 6.3.5.1) catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NADSYN1
• Uniprot:
  • P0A5L6(Bacteria)