N-PAC Knockout 293T Cell Lysate

Product Discontinued

N-PAC Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,10 bp insertion in exon 3
• Gene: GLYR1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for N-PAC Knockout 293T Cell Lysate

• BM045
• Cytokine-like nuclear factor N-PAC
• glyoxylate reductase 1 homolog (Arabidopsis)
• Glyoxylate reductase 1 homolog
• HIBDLnuclear protein 60 kDa
• NP603-hydroxyisobutyrate dehydrogenase-like protein
• N-PAC
• nuclear protein 60kDa
• Nuclear protein NP60
• Nuclear protein of 60 kDa
• putative oxidoreductase GLYR1

Background

N-PAC may have oxidoreductase activity. Regulates p38 MAP kinase activity by mediating stress activation ofp38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 andactivation of ATF2. The phosphorylation of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GLYR1