Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK His Protein

SDS-Page: Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK Protein [NBP1-99050] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-344 of Human N-Acetyl-D-Glucosamine Kinase/NAGK
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NAGK
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 39.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 200 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK His Protein

• EC 2.7.1.59
• GlcNAc Kinase
• GNK
• GNKGlcNAc kinase
• N-acetyl-D-glucosamine kinase
• N-Acetylglucosamine Kinase
• N-acetylglucosamine kinaseHSA242910
• NAGK

Background

NAGK (N-acetyl-D-glucosamine kinase) belongs to the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. It is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK has been shown to interact with STK16 and LNX1. Also it has ManNAc kinase activity. Recombinant human NAGK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NAGK