Myosin heavy chain 14 Antibody (MY-32)

Product Discontinued

Myosin heavy chain 14 Antibody (MY-32) Summary

• Immunogen: Rabbit muscle myosin.
• Specificity: NB600-701 recognizes myosin, the main constituent of the thick filaments of muscle fibres. Myosin acts with actin in the contraction and relaxation of muscles. This antibody detects skeletal muscle myosin specifically the fast twitch (type II) isomyosin molecules of the heavy chain of human or animal myosin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYH14
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:40
  • Western Blot
• Application Notes: Use in Western blot reported in scientific literature (PMID 27503321).
  Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:100-1:500. Apparent MW in kDa on Simple Western was 223kDa; matrix was 12-230 kDa.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites with PBS and 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Myosin heavy chain 14 Antibody (MY-32)

• DFNA4
• DFNA4A
• FP17425
• MHC16
• MYH14
• MYH17
• myosin, heavy chain 14, non-muscle
• NMHC II-C
• NMHC-II-C
• PNMHH

Background

Myosin is a 480 kD protein that interacts with actin in muscle and non muscle cells (8). Conventional myosin contains two identical heavy chains (MHC-200kD each) and four light chains (MLC-15-26 kD). Conventional myosin molecules consist of two major regions: tail (rod) and globular heads; they aggregate into filaments through the tail region and interact with actin and with adenosine triphosphate (ATP) through the head region. Multiple forms of myosin heavy chains exist in skeletal, smooth and cardiac muscle and in non-muscle tissues. A spectrum of skeletal muscle fiber types is found in adult skeletal muscles. The two major skeletal muscle fiber types are type I (slow-twitch) and type II (fast-twitch). Skeletal muscle type II fibers can be further subdivided into types IIa (fast red) and IIb (fast white).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin fast skeletal Antibody (NB600-701)(1)

Publication using NB600-701

• Labroo Pratima, Shea Jill, Sant Himanshu et al. Effect Of combining FK506 and neurotrophins on neurite branching and elongation. Muscle Nerve 2017-01-01 [PMID: 27503321] (WB)

Bioinformatics

• Gene Symbol: MYH14
• Entrez:
  • 79784(Human)
  • 100009542(Rabbit)
• Uniprot:
  • Q7Z406(Human)
  • P02608(Rabbit)