Myoglobin Antibody

Immunohistochemistry-Paraffin: Myoglobin Antibody [NB120-9536] - Staining of Human Skeletal Muscle.

Product Discontinued

Myoglobin Antibody Summary

• Immunogen: Myoglobin isolated from human heart.
• Localization: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.
• Specificity: This reacts with human myoglobin. The stains strongly with skeletal and cardiac muscles. No non-specific staining with other tissues has been observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:200

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS with BSA
• Preservative: Sodium Azide
• Purity: IgG purified

Alternate Names for Myoglobin Antibody

• MB
• MGC13548
• Myoglobin
• PVALB

Background

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MB
• Entrez:
  • 4151(Human)