MURF2 Antibody

Western Blot: MURF2 Antibody [NB100-1196] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Rt, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MURF2 Antibody Summary

• Description: Novus Biologicals Goat MURF2 Antibody (NB100-1196) is a polyclonal antibody validated for use in WB and ELISA. Anti-MURF2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HIFSFSWLNSLNE corresponding to C-Terminus according to NP_908974.1, NP_908975.1.
• Specificity: This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).
• Predicted Species: Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM55
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 85 kDa band observed in human and mouse heart lysates is reported in scientific literature (PMID: 17272810).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MURF2 Antibody

• muRF2
• MURF-2
• Muscle-specific RING finger protein 2
• RING finger protein 29muscle specific ring finger 2
• RNF29
• tripartite motif containing 55
• tripartite motif-containing 55
• tripartite motif-containing protein 55

Background

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein associates transiently with microtubules, myosin, and titin during muscle sarcomere assembly. It may act as a transient adaptor and plays a regulatory role in the assembly of sarcomeres. Four alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MURF2 Antibody (NB100-1196)(2)

Publications using NB100-1196

• Willis MS, Ike C, Li L et al. Muscle ring finger 1, but not muscle ring finger 2, regulates cardiac hypertrophy in vivo. Circ Res 2007-03-01 [PMID: 17272810]
• Centner T, Yano J, Kimura E et al. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J Mol Biol 2001-03-02 [PMID: 11243782]

Bioinformatics

• Gene Symbol: TRIM55
• Entrez:
  • 84675(Human)
  • 381485(Mouse)
  • 365751(Rat)
• Uniprot:
  • NP_149047.(Human)