MURF2 Antibody

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Western Blot: MURF2 Antibody [NB100-1196] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MURF2 Antibody Summary

Immunogen
Peptide with sequence C-HIFSFSWLNSLNE corresponding to C-Terminus according to NP_908974.1, NP_908975.1.
Specificity
This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM55
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 85 kDa band observed in human and mouse heart lysates is reported in scientific literature (PMID: 17272810).
Publications
Read Publications using NB100-1196.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for MURF2 Antibody

  • muRF2
  • MURF-2
  • Muscle-specific RING finger protein 2
  • RING finger protein 29muscle specific ring finger 2
  • RNF29
  • tripartite motif containing 55
  • tripartite motif-containing 55
  • tripartite motif-containing protein 55

Background

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein associates transiently with microtubules, myosin, and titin during muscle sarcomere assembly. It may act as a transient adaptor and plays a regulatory role in the assembly of sarcomeres. Four alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MURF2 Antibody (NB100-1196)(2)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM55
Entrez
Uniprot