MUC15 Overexpression Lysate (Native)

Images

 
Western Blot: MUC15 Overexpression Lysate (Adult Normal) [NBP2-09564] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MUC15.

Product Details

Summary
Applications WB

Order Details

MUC15 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001128564. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens MUC15, mRNA.
Gene
MUC15

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
33.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MUC15

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MUC15 Overexpression Lysate (Native)

  • MUC-15
  • mucin 15, cell surface associated
  • mucin-15
  • PAS3
  • PASIII

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-52193
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-92151
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-60046
Species: Hu, Mu, Rt, Po, Bv, Eq, Gt, GP, Rb
Applications: WB, ICC/IF, IHC, IHC-P
NB120-11197
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-Fr, IHC-P
NBP2-24566
Species: Hu
Applications: WB, IHC-P
NB200-124
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
NB600-1468
Species: Hu
Applications: WB, IHC-P, S-ELISA
NBP2-15196
Species: Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)
Applications: Flow, ICC/IF, IHC-Fr, IHC-P, Flow-IC
NBP1-91013
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-82045
Species: Hu
Applications: IHC, IHC-P
NBP2-44432
Species: Hu
Applications: Flow, IHC-P, PAGE, IF
NBP2-44376
Species: Hu, Rt(-)
Applications: WB, Flow, IHC-P, IF
MAB8245
Species: Hu
Applications: IHC
H00200958-P01
Species: Hu
AF932
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NBP1-86210
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P
NBP1-79720
Species: Hu
Applications: WB
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for MUC15 Lysate (NBP2-09564) (0)

There are no publications for MUC15 Lysate (NBP2-09564).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MUC15 Lysate (NBP2-09564) (0)

There are no reviews for MUC15 Lysate (NBP2-09564). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MUC15 Lysate (NBP2-09564) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MUC15 Products

Bioinformatics Tool for MUC15 Lysate (NBP2-09564)

Discover related pathways, diseases and genes to MUC15 Lysate (NBP2-09564). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MUC15 Lysate (NBP2-09564)

Discover more about diseases related to MUC15 Lysate (NBP2-09564).
 

Pathways for MUC15 Lysate (NBP2-09564)

View related products by pathway.

PTMs for MUC15 Lysate (NBP2-09564)

Learn more about PTMs related to MUC15 Lysate (NBP2-09564).

Blogs on MUC15

There are no specific blogs for MUC15, but you can read our latest blog posts.

Customers Who Bought This Also Bought

MUC15 Antibody
NBP1-86406
MUC-1 Antibody
NBP1-60046
MUC-1 Antibody
NBP1-60046
MUC-1 Antibody
NBP1-60046

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MUC15 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol MUC15