mtRNA polymerase Antibody - BSA Free

Western Blot: mtRNA polymerase Antibody [NBP3-18334] - WB Validation in Human Cell Lines. Loading: 15 ug of lysate. Antibodies: mtRNA polymerase, 2 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunocytochemistry/Immunofluorescence: mtRNA polymerase Antibody [NBP3-18334] - Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling mtRNA polymerase with NBP3-18334 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody - BSA Free [NBP3-18334] - Validation of POLRMT in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling POLRMT with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody - BSA Free [NBP3-18334] - Validation of POLRMT in Mouse Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling POLRMT with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• Reactivity: Hu, Mu, Rt, Pm, Fe
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

mtRNA polymerase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit mtRNA polymerase Antibody - BSA Free (NBP3-18334) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human mtRNA polymerase. The immunogen is located within the last 50 amino acids of mtRNA polymerase.
• Predicted Species: Chimpanzee (100%), Feline (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLRMT
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Western Blot 2 ug/mL
• Control Peptide:

  mtRNA polymerase Protein (NBP3-18334PEP)

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine (82%); Monkey (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for mtRNA polymerase Antibody - BSA Free

• APOLMTEC 2.7.7.6
• DNA-directed RNA polymerase, mitochondrial
• EC 2.7.7.6
• h-mtRPOL
• mtRNA polymerase
• MTRNAP
• MtRPOL
• POLRMT
• polymerase (RNA) mitochondrial (DNA directed)

Background

POLRMT, also known as DNA-directed RNA polymerase, mitochondrial, is a 246 amino acid protein that is 28 kDa, catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. This protein is currently being studied for research on autosomal dominant progressive external ophthalmoplegia, essential thrombocythemia, ophthalmoplegia, immunodeficiency, and malaria. This protein has also shown to have interactions with TFB1M, NFKB2, ICT1, TFB2M, TFAM, and over 200 other proteins in ATP/ITP metabolism, mitochondrial gene expression, transcription from mitochondrial promoters, transcription, RNA polymerase I, RNA polymerase III, and mitochondrial transcription, gene expression, and mitochondrial transcription initiation pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLRMT