mtRNA polymerase Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Mu, Rt, Pm, Fe
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

mtRNA polymerase Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Antibody was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human mtRNA polymerase. The immunogen is located within the last 50 amino acids of mtRNA polymerase.
• Predicted Species: Chimpanzee (100%), Feline (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLRMT
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine (82%); Monkey (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Peptide affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for mtRNA polymerase Antibody [CoraFluor™ 1]

• APOLMTEC 2.7.7.6
• DNA-directed RNA polymerase, mitochondrial
• EC 2.7.7.6
• h-mtRPOL
• mtRNA polymerase
• MTRNAP
• MtRPOL
• POLRMT
• polymerase (RNA) mitochondrial (DNA directed)

Background

POLRMT, also known as DNA-directed RNA polymerase, mitochondrial, is a 246 amino acid protein that is 28 kDa, catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. This protein is currently being studied for research on autosomal dominant progressive external ophthalmoplegia, essential thrombocythemia, ophthalmoplegia, immunodeficiency, and malaria. This protein has also shown to have interactions with TFB1M, NFKB2, ICT1, TFB2M, TFAM, and over 200 other proteins in ATP/ITP metabolism, mitochondrial gene expression, transcription from mitochondrial promoters, transcription, RNA polymerase I, RNA polymerase III, and mitochondrial transcription, gene expression, and mitochondrial transcription initiation pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLRMT