MTF1 Antibody (MTF1/2649)

Immunohistochemistry-Paraffin: MTF1 Antibody (MTF1/2649) [NBP3-07236] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with MTF1 Mouse Monoclonal Antibody (MTF1/2649).

• Reactivity: Hu
• Applications: IHC
• Clone: MTF1/2649
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MTF1 Antibody (MTF1/2649) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08238)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human MTF1 protein (Uniprot: Q14872)
• Localization: Nucleus.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MTF1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for MTF1 Antibody (MTF1/2649)

• metal regulatory transcription factor 1
• metal-regulatory transcription factor 1
• metal-responsive transcription factor 1
• MGC23036
• MRE-binding transcription factor
• MRE-binding transcription factor-1
• MTF-1
• Transcription factor MTF-1
• zinc regulatory factor
• ZRF

Background

MTF1 encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MTF1
• Uniprot:
  • Q14872(Human)