MST3 Antibody - BSA Free

Western Blot: MST3 Antibody [NBP1-31360] - A. 50 ug rat brain lysate/extract 10 % SDS-PAGE STK24 antibody [N2C1], Internal dilution: 1:1000

Immunocytochemistry/Immunofluorescence: MST3 Antibody [NBP1-31360] - Analysis of HeLa, using NBP1-31360 at 1:200 dilution.

Immunohistochemistry-Paraffin: MST3 Antibody [NBP1-31360] - Paraffin-embedded human breast carcinoma. STK24 antibody [N2C1], Internal dilution: 1:250.

Western Blot: MST3 Antibody [NBP1-31360] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Rt, Mu, Bv, Ch, RM, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MST3 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MST3. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Mouse (100%), Rhesus Macaque (100%), Bovine (100%), Xenopus (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STK24
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MST3 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• Mammalian STE20-like protein kinase 3
• MST-3
• MST3serine/threonine kinase 24 (Ste20, yeast homolog)
• serine/threonine kinase 24
• serine/threonine-protein kinase 24
• STE20
• STE20-like kinase 3
• STE20-like kinase MST3
• sterile 20-like kinase 3
• STK3yeast)

Background

The growth of axons is fundamental to the development and repair of brain circuitry. It has been shown that Mst3, a neuron-specific homolog of the yeast kinase Ste20, is critical for axon outgrowth. Mst3 is activated in response to trophic factors, and suppressing its expression or its function blocks axon outgrowth (1). Mst3 has been recently demonstrated to undergo a caspase-mediated cleavage during apoptosis. The proteolytic cleavage of the C-terminus of Mst3 caused nuclear translocation of its kinase domain. Mst3 contains both nuclear localization sequence (NLS) and NES signals, which may cooperate to control the subcellular distribution of Mst3 (2). In situ hybridization of rat brain sections indicated that MST3b is widely expressed in different brain regions, with especially high expression in hippocampus and cerebral cortex. When expressed in human embryonic kidney 293 (HEK293) cells, MST3b effectively phosphorylated myelin basic protein, as well as undergoing autophosphorylation (3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: STK24