MST3 Antibody

Images

 
Western Blot: MST3 Antibody [NBP1-31360] - A. 50 ug rat brain lysate/extract 10 % SDS-PAGE STK24 antibody [N2C1], Internal dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: MST3 Antibody [NBP1-31360] - Analysis of HeLa, using NBP1-31360 at 1:200 dilution.
Immunohistochemistry-Paraffin: MST3 Antibody [NBP1-31360] - Paraffin-embedded human breast carcinoma. STK24 antibody [N2C1], Internal dilution: 1:250.
Western Blot: MST3 Antibody [NBP1-31360] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Rt, Mu, Bv, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.94 mg/ml

Order Details

MST3 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human STK24. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Mouse (100%), Rhesus Macaque (100%), Bovine (100%), Xenopus (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STK24
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.94 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MST3 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • Mammalian STE20-like protein kinase 3
  • MST-3
  • MST3serine/threonine kinase 24 (Ste20, yeast homolog)
  • serine/threonine kinase 24
  • serine/threonine-protein kinase 24
  • STE20
  • STE20-like kinase 3
  • STE20-like kinase MST3
  • sterile 20-like kinase 3
  • STK3yeast)

Background

The growth of axons is fundamental to the development and repair of brain circuitry. It has been shown that Mst3, a neuron-specific homolog of the yeast kinase Ste20, is critical for axon outgrowth. Mst3 is activated in response to trophic factors, and suppressing its expression or its function blocks axon outgrowth (1). Mst3 has been recently demonstrated to undergo a caspase-mediated cleavage during apoptosis. The proteolytic cleavage of the C-terminus of Mst3 caused nuclear translocation of its kinase domain. Mst3 contains both nuclear localization sequence (NLS) and NES signals, which may cooperate to control the subcellular distribution of Mst3 (2). In situ hybridization of rat brain sections indicated that MST3b is widely expressed in different brain regions, with especially high expression in hippocampus and cerebral cortex. When expressed in human embryonic kidney 293 (HEK293) cells, MST3b effectively phosphorylated myelin basic protein, as well as undergoing autophosphorylation (3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK24