Human MSP/MST1 ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1752
    • Availability
      Product Discontinued

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Human MSP/MST1 ELISA Kit (Colorimetric) Summary

Sensitivity
8 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MST1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
MST1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MST1.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): Diluent buffer containing 0.09% sodium azide aspreservative.
  2. Assay Diluent B (Item E): 5x concentrated buffer.
  3. Detection Antibody MSP (Item F): Biotinylated anti-human MSP. Each vial is enough toassay half the microplate.
  4. HRP-Streptavidin Concentrate (Item G): 200x concentrated HRP-conjugatedStreptavidin.
  5. MSP Microplate (Item A): Coated with anti-human MSP.
  6. Note: Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
  7. Standard Protein (Item C): Human MSP. 1 vial is enough to run each standard induplicate.
  8. Stop Solution (Item I): 0.2 M sulfuric acid.
  9. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’- tetramethylbenzidine (TMB) inbuffered solution.
  10. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human MSP/MST1 ELISA Kit (Colorimetric)

  • D3F15S2
  • EC 3.4.21
  • hepatocyte growth factor-like protein homolog
  • HGFL
  • HGFLhepatocyte growth factor-like protein
  • macrophage stimulating 1 (hepatocyte growth factor-like)
  • Macrophage stimulatory protein
  • Macrophage-stimulating protein
  • MSP
  • MSPDNF15S2
  • MST1
  • NF15S2
  • SF2

Background

Mammalian STE20-like kinase 1, or MST-1, is a serine/threonine kinase that, upon cleavage, has been implicated in the promotion of chromatin condensation. MST-1 is also known as KRS2 and STK4. The C-terminus of MST-1 contains two functional NESs (nuclear export signals), which are released upon caspase-mediated cleavage. The N-terminus portion of the protein then translocates to the nucleus and will promote chromatin condensation at sufficiently high levels. Full-length MST-1 is localized to the cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MST1