Human MSP/MST1 ELISA Kit (Colorimetric) Summary
Sensitivity |
8 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 12% |
Intra-Assay |
CV% < 10% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
MST1 |
Applications/Dilutions
Dilutions |
|
Application Notes |
MST1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MST1. |
Reactivity Notes
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Assay Diluent A (Item D): Diluent buffer containing 0.09% sodium azide aspreservative.
- Assay Diluent B (Item E): 5x concentrated buffer.
- Detection Antibody MSP (Item F): Biotinylated anti-human MSP. Each vial is enough toassay half the microplate.
- HRP-Streptavidin Concentrate (Item G): 200x concentrated HRP-conjugatedStreptavidin.
- MSP Microplate (Item A): Coated with anti-human MSP.
- Note: Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
- Standard Protein (Item C): Human MSP. 1 vial is enough to run each standard induplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’- tetramethylbenzidine (TMB) inbuffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human MSP/MST1 ELISA Kit (Colorimetric)
Background
Mammalian STE20-like kinase 1, or MST-1, is a serine/threonine kinase that, upon cleavage, has been implicated in the promotion of chromatin condensation. MST-1 is also known as KRS2 and STK4. The C-terminus of MST-1 contains two functional NESs (nuclear export signals), which are released upon caspase-mediated cleavage. The N-terminus portion of the protein then translocates to the nucleus and will promote chromatin condensation at sufficiently high levels. Full-length MST-1 is localized to the cytoplasm.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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