MSL3L1 Antibody

Western Blot: MSL3L1 Antibody [NBP2-19419] - Sample (50 ug of whole cell lysate) A: Mouse Muscle, 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: MSL3L1 Antibody [NBP2-19419] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MSL3L1 protein stained by MSL3L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: MSL3L1 Antibody [NBP2-19419] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:500.

Immunoprecipitation: MSL3L1 Antibody [NBP2-19419] - MSL3L1 antibody immunoprecipitates MSL3L1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract. A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of MSL3L1 protein by 2 ug of MSL3L1 antibody. 7.5% SDS-PAGE. The immunoprecipitated MSL3L1 protein was detected by MSL3L1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MSL3L1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MSL3L1. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSL3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MSL3L1 Antibody

• male-specific lethal 3 homolog (Drosophila)
• male-specific lethal 3 homolog
• male-specific lethal 3-like 1 (Drosophila)
• male-specific lethal-3 (Drosophila)-like 1
• Male-specific lethal-3 homolog 1
• Male-specific lethal-3 protein-like 1
• MSL3L1DKFZp586J1822
• MSL3-like 1

Background

This gene encodes a nuclear protein and has similarity to drosophila male-specific lethal-3 gene. The drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus this encoded protein is thought to play a similar function in chromatin remodeling and transcriptional regulation. This gene has been found to undergo X inactivation. There are four alternatively spliced transcript variants of this gene encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MSL3
• Uniprot:
  • Q8N5Y2(Human)