MSK1/RPS6KA5 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse MSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2518) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK1 was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MSK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse MSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2518) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MSK1/RPS6KA5 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MSK1
  Met1-Ala802
  Accession # O75582
• Specificity: Detects human and mouse MSK1 in Western blots. This antibody does not detect recombinant human MSK2.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RPS6KA5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSK1/RPS6KA5 Antibody [Unconjugated]

• EC 2.7.11
• MGC1911,90 kDa ribosomal protein S6 kinase 5
• MSK1
• MSK1EC 2.7.11.1
• MSPK1
• Nuclear mitogen- and stress-activated protein kinase 1
• ribosomal protein S6 kinase alpha-5
• ribosomal protein S6 kinase, 90kD, polypeptide 5
• ribosomal protein S6 kinase, 90kDa, polypeptide 5
• RLPK
• RPS6KA5
• RSKL
• RSK-like protein kinase
• S6K-alpha-5

Background

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2/RSKB have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSK1/RPS6KA5 Antibody (AF2518)(10)

Publications using AF2518

• L Gresseau, ME Roy, S Duhamel, B Annabi A Signaling Crosstalk Links SNAIL to the 37/67 kDa Laminin-1 Receptor Ribosomal Protein SA and Regulates the Acquisition of a Cancer Stem Cell Molecular Signature in U87 Glioblastoma Neurospheres Cancers, 2022-11-30;14(23):. 2022-11-30 [PMID: 36497426] (Western Blot, Human)
• M Hou, W Wang, F Hu, Y Zhang, D Yang, Q Liu Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner Infect. Immun., 2018-12-19;87(1):. 12/19/2018 [PMID: 30396897]
• Judit M. Pérez Ortiz, Nissa Mollema, Nicholas Toker, Carolyn J. Adamski, Brennon O'Callaghan, Lisa Duvick et al. Reduction of protein kinase A-mediated phosphorylation of ATXN1-S776 in Purkinje cells delays onset of Ataxia in a SCA1 mouse model Neurobiology of Disease 2018-08-01 [PMID: 29758256]
• Camille Danne, Grigory Ryzhakov, Maria Martínez-López, Nicholas Edward Ilott, Fanny Franchini, Fiona Cuskin et al. A Large Polysaccharide Produced by Helicobacter hepaticus Induces an Anti-inflammatory Gene Signature in Macrophages Cell Host & Microbe 2017-12-13 [PMID: 29241040]
• Alejandro Huante-Mendoza, Octavio Silva-García, Javier Oviedo-Boyso, Robert E. W. Hancock, Víctor M. Baizabal-Aguirre Peptide IDR-1002 Inhibits NF-kappa B Nuclear Translocation by Inhibition of I kappa B alpha Degradation and Activates p38/ERK1/2–MSK1-Dependent CREB Phosphorylation in Macrophages Stimulated with Lipopolysaccharide Frontiers in Immunology 2016-11-25 [PMID: 27933067]
• Habiba Harouz, Christophe Rachez, Benoit M Meijer, Benoit Marteyn, Françoise Donnadieu, Florence Cammas et al. Shigella flexneri targets the HP1 gamma subcode through the phosphothreonine lyase OspF The EMBO Journal 2014-11-18 [PMID: 25216677]
• Jeehye Park, Ismael Al-Ramahi, Qiumin Tan, Nissa Mollema, Javier R. Diaz-Garcia, Tatiana Gallego-Flores et al. Genetic screens reveal RAS/MAPK/MSK1 modulate ataxin 1 protein levels and toxicity in SCA1 Nature 2013-06-20 [PMID: 23719381]
• Dreses-Werringloer U, Vingtdeux V, Zhao H, Chandakkar P, Davies P, Marambaud P CALHM1 controls the Ca(2)(+)-dependent MEK, ERK, RSK and MSK signaling cascade in neurons. J Cell Sci, 2013-01-23;126(0):1199-206. 2013-01-23 [PMID: 23345406] (Western Blot, Mouse)
• Chen D, Bambah-Mukku D, Pollonini G, Alberini C Glucocorticoid receptors recruit the CaMKIIalpha-BDNF-CREB pathways to mediate memory consolidation. Nat Neurosci, 2012-11-18;15(12):1707-14. 2012-11-18 [PMID: 23160045] (Western Blot, Rat)
• Sindreu CB, Scheiner ZS, Storm DR Ca2+ -stimulated adenylyl cyclases regulate ERK-dependent activation of MSK1 during fear conditioning. Neuron, 2007-01-04;53(1):79-89. 2007-01-04 [PMID: 17196532] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: RPS6KA5
• Uniprot:
  • O75582()