MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-08282] - Immunofluorescence Analysis of HeLa cells using MSK1/RPS6KA5 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-08282] - Flow Cytometric Analysis of PFA-fixed HeLa cells. MSK1/RPS6KA5 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).

Protein Array: MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) [NBP3-08282] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MSK1/RPS6KA5 Mouse Monoclonal Antibody (PCRP-RPS6KA5-1A8).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, MA
• Clone: PCRP-RPS6KA5-1A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07556).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human MSK1/RPS6KA5 protein (Uniprot: O75582)
• Localization: Nucleus, Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS6KA5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for MSK1/RPS6KA5 Antibody (PCRP-RPS6KA5-1A8) - Azide and BSA Free

• EC 2.7.11
• MGC1911,90 kDa ribosomal protein S6 kinase 5
• MSK1
• MSK1EC 2.7.11.1
• MSPK1
• Nuclear mitogen- and stress-activated protein kinase 1
• ribosomal protein S6 kinase alpha-5
• ribosomal protein S6 kinase, 90kD, polypeptide 5
• ribosomal protein S6 kinase, 90kDa, polypeptide 5
• RLPK
• RPS6KA5
• RSKL
• RSK-like protein kinase
• S6K-alpha-5

Background

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA5