MSK1/RPS6KA5 Antibody (2B11) - Azide and BSA Free

Western Blot: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01] - Analysis of RPS6KA5 expression in transfected 293T cell line by RPS6KA5 monoclonal antibody (M01), clone 2B11.Lane 1: RPS6KA5 transfected lysate(89.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01] - Analysis of monoclonal antibody to RPS6KA5 on HeLa cell . Antibody concentration 10 ug/ml.

Western Blot: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01] - RPS6KA5 monoclonal antibody (M01), clone 2B11. Western Blot analysis of RPS6KA5 expression in HeLa ( Cat # L013V1 ).

Proximity Ligation Assay: MSK1/RPS6KA5 Antibody (2B11) [H00009252-M01] - Analysis of protein-protein interactions between NR4A1 and RPS6KA5. HeLa cells were stained with anti-NR4A1 rabbit purified polyclonal 1:1200 and anti-RPS6KA5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF
• Clone: 2B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MSK1/RPS6KA5 Antibody (2B11) - Azide and BSA Free Summary

• Immunogen: RPS6KA5 (AAH17187.1, 271 a.a. ~ 370 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ILKSEPPYPQEMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHLFFQKINWDDLAAKKVPAPFKPVIRDELDVSNFAEEFTEMDPTYSPAALPQSSEK
• Specificity: RPS6KA5 - ribosomal protein S6 kinase, 90kDa, polypeptide 5 (2B11)
• Isotype: IgG2b Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS6KA5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MSK1/RPS6KA5 Antibody (2B11) - Azide and BSA Free

• EC 2.7.11
• MGC1911,90 kDa ribosomal protein S6 kinase 5
• MSK1
• MSK1EC 2.7.11.1
• MSPK1
• Nuclear mitogen- and stress-activated protein kinase 1
• ribosomal protein S6 kinase alpha-5
• ribosomal protein S6 kinase, 90kD, polypeptide 5
• ribosomal protein S6 kinase, 90kDa, polypeptide 5
• RLPK
• RPS6KA5
• RSKL
• RSK-like protein kinase
• S6K-alpha-5

Background

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA5
• Entrez:
  • 9252(Human)
• Uniprot:
  • AAH17187(Human)