MSH2 Antibody (10G1)

Western Blot: MSH2 Antibody (10G1) [NBP2-76895] - Western blot analysis of MSH2 on K562 cells lysates using anti-MSH2 antibody.Lane 1: Anti-MSH2 antibody, 1:500.Lane 2: Anti-MSH2 antibody, 1:5,000.

Immunohistochemistry-Paraffin: MSH2 Antibody (10G1) [NBP2-76895] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MSH2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30

Immunohistochemistry: MSH2 Antibody (10G1) [NBP2-76895] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MSH2 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Immunohistochemistry: MSH2 Antibody (10G1) [NBP2-76895] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MSH2 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Immunohistochemistry: MSH2 Antibody (10G1) [NBP2-76895] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MSH2 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Immunohistochemistry-Paraffin: MSH2 Antibody (10G1) [NBP2-76895] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MSH2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5%

Product Discontinued

MSH2 Antibody (10G1) Summary

• Immunogen: Synthetic peptide within N-terminal human MSH2. (SwissProt: P43246 Human; SwissProt: P54275 Rat)
• Localization: Nucleus, Chromosome.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSH2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:2000-1:5000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for MSH2 Antibody (10G1)

• COCA1
• DNA mismatch repair protein Msh2
• FCC1
• hMSH2
• HNPCC
• HNPCC1
• HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
• LCFS2
• MSH2
• mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• MutS protein homolog 2

Background

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSH2