MS4A6A Overexpression Lysate (Native)

Images

 
Western Blot: MS4A6A Overexpression Lysate (Adult Normal) [NBL1-13320] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MS4A6A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MS4A6A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204757 and based on accession number NM_152852. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens membrane-spanning 4-domains, subfamily A, member 6A (MS4A6A), transcript variant 1, mRNA.
Gene
MS4A6A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
26.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBL1-13320 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MS4A6A Overexpression Lysate (Native)

  • 4SPAN3
  • CD20 antigen-like 3
  • CD20L3
  • CD20L3MS4A6A-polymorph
  • CD20-like precusor
  • CDA01
  • Four-span transmembrane protein 3
  • four-span transmembrane protein 3.1,4SPAN3.2
  • four-span transmembrane protein 3.2
  • HAIRB-iso
  • membrane-spanning 4-domains subfamily A member 6A
  • membrane-spanning 4-domains, subfamily A, member 6A
  • MGC131944
  • MGC22650
  • MS4A6A
  • MS4A6MST090
  • MST090
  • MSTP090

Background

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. The gene encoding this protein is localized to 11q12.1, among a cluster of family members. Alternative splicing of this gene results in several transcript variants. Transcript Variant: This variant (1) represents the longest transcript variant and encodes the longest isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MS4A6A Lysate (NBL1-13320) (0)

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Review for MS4A6A Lysate (NBL1-13320) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBL1-13320:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
sean taheri
WB Human 05/26/2010
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Summary

ApplicationWestern Blot
Sample Testedmesothelin lysate, Sample Amount: 20 ug
SpeciesHuman
CommentsRan a western looking for MSLN. Used Novus NBL1-1332 ( 20 ug) as positive control. results were clean.

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBS, Blocking Time: 2 hours, Blocking Temp: 4 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: AB6721, Secondary Ab Dilution Ratio: 1:1000
Secondary Manufacturer Cat#AB6721

Details

Detection NotesECL, Exposure Time:10min, Pos Ctrl:MCF-7 whole cell lysate, Neg Ctrl:LNCAp, Bands:Specific=69kDa, Non-Specific=55kDa

Comments

CommentsRan a western looking for MSLN. Used Novus NBL1-1332 ( 20 ug) as positive control. results were clean.

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FAQs for MS4A6A Lysate (NBL1-13320) (0)

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Diseases for MS4A6A Lysate (NBL1-13320)

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Recent Reviews

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sean taheri
05/26/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol MS4A6A
Entrez