MS4A6A Overexpression Lysate

Western Blot: MS4A6A Overexpression Lysate (Adult Normal) [NBL1-13320] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MS4A6A.

• Reactivity: Hu
• Applications: WB

MS4A6A Overexpression Lysate Summary

• Description:

  MS4A6A Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC204757
  
  Accession#: NM_152852
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).
• Gene: MS4A6A

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.
• Theoretical MW: 26.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for MS4A6A Overexpression Lysate

• 4SPAN3
• CD20 antigen-like 3
• CD20L3
• CD20L3MS4A6A-polymorph
• CD20-like precusor
• CDA01
• Four-span transmembrane protein 3
• four-span transmembrane protein 3.1,4SPAN3.2
• four-span transmembrane protein 3.2
• HAIRB-iso
• membrane-spanning 4-domains subfamily A member 6A
• membrane-spanning 4-domains, subfamily A, member 6A
• MGC131944
• MGC22650
• MS4A6A
• MS4A6MST090
• MST090
• MSTP090

Background

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. The gene encoding this protein is localized to 11q12.1, among a cluster of family members. Alternative splicing of this gene results in several transcript variants. Transcript Variant: This variant (1) represents the longest transcript variant and encodes the longest isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Review for MS4A6A Lysate (NBL1-13320) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: sean taheri WB Human 05/26/2010

Summary

• Application: Western Blot
• Sample Tested: mesothelin lysate, Sample Amount: 20 ug
• Species: Human

Bioinformatics

• Gene Symbol: MS4A6A