MRPS35 Antibody - Azide and BSA Free

Western Blot: MRPS35 Antibody [NBP2-94003] - Analysis of extracts of various cell lines, using MRPS35 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.

Immunocytochemistry/Immunofluorescence: MRPS35 Antibody [NBP2-94003] - Analysis of L929 cells using MRPS35 at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: MRPS35 Antibody [NBP2-94003] - Human colon carcinoma using MRPS35 Rabbit pAb at dilution of 1:100 (40x lens).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MRPS35 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit MRPS35 Antibody - Azide and BSA Free (NBP2-94003) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human MRPS35 (NP_068593.2). MAAAALPAWLSLQSRARTLRAFSTAVYSATPVPTPSLPERTPGNERPPRRKALPPRTEKMAVDQDWPSVYPVAAPFKPSAVPLPVRMGYPVKKGVPMAKEGNLELLKIPNFLHLTPVAIKKHCEALKDFCTEWPAALDSDEKCEKHFPIEIDSTDYVSSGPSVRNPRARVVVLRVKLSSLNLDDHAKKKLIKLVGERYCKTTDVLTIKTDRCPLRRQNYDYAVYLLTVLYHESWNTEEWEKSKTEADMEEYIWENSSSERNILETLLQMKAAEKNMEINKEELLGTKEIEEYKKSVVSLKNEEENENSISQYKESVKRLLNVT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPS35
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for MRPS35 Antibody - Azide and BSA Free

• 28S ribosomal protein S28, mitochondrial
• DKFZp762P093
• MDS023,28S ribosomal protein S35, mitochondrial
• MGC104278
• mitochondrial ribosomal protein S28
• mitochondrial ribosomal protein S35
• MRP-S28
• MRPS28S35mt
• MRP-S35
• S28mt

Background

MRPS35, or Mitochondrial Ribosomal Protein S35, contains a 37 kDa and a 33 kDa isoform, and is involved in protein synthesis within the mitochondria. The protein is not currently being used in any disease research. MRPS35 has been linked to the DNA damage response and the peptide biosynthetic process, through which it interacts with several other proteins, including ICT1, USP42, UBC, ESR1, and B9D1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: MRPS35