MRPL12 Antibody - BSA Free

Western Blot: MRPL12 Antibody [NBP2-19397] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-19397] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody [NBP2-19397] - MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.Green: MRPL12 protein stained by MRPL12 antibody [N1C3] (NBP2-19397) diluted at 1:500.Blue: Hoechst 33342 staining.

Western Blot: MRPL12 Antibody [NBP2-19397] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with MRPL12 antibody [N1C3] (NBP2-19397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPL12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MRPL12 Antibody - BSA Free (NBP2-19397) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MRPL12. The exact sequence is proprietary.
• Localization: Mitochondrion
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL12
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MRPL12 Antibody - BSA Free

• 5c5-2
• FLJ60124
• L12mt
• MGC8610
• mitochondrial ribosomal protein L12
• MRP-L12
• MRP-L31/34
• MRPL7
• MRPL7/L12
• RPML1239S ribosomal protein L12, mitochondrial

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MRPL12
• Uniprot:
  • P52815(Human)