MPP3 Antibody (4D7) - Azide and BSA Free

Western Blot: MPP3 Antibody (4D7) [H00004356-M01] - Analysis of MPP3 expression in transfected 293T cell line by MPP3 monoclonal antibody (M01), clone 4D7.Lane 1: MPP3 transfected lysate(66.2 KDa).Lane 2: Non-transfected lysate.

Western Blot: MPP3 Antibody (4D7) [H00004356-M01] - Analysis of MPP3 over-expressed 293 cell line, cotransfected with MPP3 Validated Chimera RNAi ( Cat # H00004356-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP3 monoclonal antibody (M01), clone 4D7 (Cat # H00004356-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: MPP3 Antibody (4D7) [H00004356-M01] - Detection limit for recombinant GST tagged MPP3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 4D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MPP3 Antibody (4D7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MPP3 (AAH56865, 1 a.a. ~ 585 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPVLSEDSGLHETLALLTSQLRPDSNHKEEMGFLRDVFSEKSLSYLMKIHEKLRYYERQSPTPVLHSAVALAEDVMEELQAASVHSDERELLQLLSTPHLRAVLMVHDTVAQKNFDPVLPPLPDNIDEDFDEESVKIVRLVKNKEPLGATIRRDEHSGAVVVARIMRGGAADRSGLVHVGDELREVNGIAVLHKRPDEISQILAQSQGSITLKIIPATQEEDRLKESKVFMRALFHYNPREDRAIPCQEAGLPFQRRQVLEVVSQDDPTWWQAKRVGDTNLRAGLIPSKGFQERRLSYRRAAGTLPSPQSLRKPPYDQPCDKETCDCEGYLKGHYVAGLRRSFRLGCRERLGGSQEGKMSSGAESPELLTYEEVARYQHQPGERPRLVVLIGSLGARLHELKQKVVAENPQHFGVAVPHTTRPRKSHEKEGVEYHFVSKQAFEADLHHNKFLEHGEYKENLYGTSLEAIQAVMAKNKVCLVDVEPEALKQLRTSEFKPYIIFVKPAIQEKRKTPPMSPACEDTAAPFDEQQQEMAASAAFIDRHYGHLVDAVLVKEDLQGAYSQLKVVLEKLSKDTHWVPVSWVR
• Specificity: MPP3 - membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPP3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MPP3 Antibody (4D7) - Azide and BSA Free

• Discs large homolog 3
• discs, large homolog 3
• DLG3MAGUK p55 subfamily member 3
• membrane protein palmitoylated 3
• membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
• Protein MPP3

Background

This gene product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MPP3
• Entrez:
  • 4356(Human)
• OMIM:
  • 601114(Human)
• Uniprot:
  • AAH56865(Human)